신 사구체 질환에서 Nephrin, Glomerular Epithelial Cell Protein-1 [GLEPP1]
Altered Expression of Nephrin, Glomerular Epithelial Cell Protein-1, (GLEPP1) and WT-1 in Glomerular Disease
김병권, 김지훈, 이현순,
소속 상세정보
김병권 ( Kim Byoung-Kwon )
서울대학교 의과대학 병리학교실
김지훈 ( Kim Ji-Hoon )
서울대학교 의과대학 병리학교실
이현순 ( Lee Hyun-Soon )
서울대학교 의과대학 병리학교실
KMID : 0357920020360010021
Abstract
Background: Glomerular epithelial cell protein-1 (GLEPP1)and WT-1 expressed in mature visceral glomerular epithelial cell(VGEC)is required for maintenance of the mature status of VGEC. Nephrin protein is located at the filtration slit and regarded as a molecular component of the slit diaphragm. Alterations of these proteins in proteinuric diseases are not clearlydefined.
Methods: We investigated the expression of GLEPP1, WT-1 and nephrin in 28 renal biopsies diagnosed with minimal change nephropathy (n=10), focal glomerulosclerosis(n=10) and membranous nephritis (n=8) by immunohistochemical staining. Normal control biopsies were obtained from six nephrectomy specimens.
Results: The patients consisted of 15 males and 13 females. The mean age was 40.7 years. Nephrotic range proteinuria (≥3.5 g/day) was noted in 15(54%) patients. GLEPP1 and nephrin expression were significantly decreased in patients as compared with those of the controls (p＜0.05). The mean number of WT-1 expressing cells per glomerulus was also significantly decreased in patients as compared with those of the controls (p＜0.05). However, there was no significant difference in the number of WT-1 expressing cells among the disease groups.
Conclusions: These results suggest that the loss of biological markers of mature VGEC may play an important role in the pathogenesis of proteinuria.
키워드
Nephrosis;Lipoid;Glomerulo Nephritis;Biological Markers
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