파라핀 포매조직을 이용한 유방암에서의 BRCA1과 BRCA2의 유전체 소실 연구
Alleic Loss at the BRCA1 and BRCA2 Loci iin Sporadic Breast Carcinoma Using Paraffin Embedded Tissue
박지영, 이명훈, 김동자, 박태인, 이영하, 김정완, 손윤경,
소속 상세정보
박지영 ( Park Ji-Young )
경북대학교 의과대학 병리학교실
이명훈 ( Lee Myung-Hoon )
경북대학교 의과대학 병리학교실
김동자 ( Kim Dong-Ja )
경북대학교 의과대학 병리학교실
박태인 ( Park Tae-In )
경북대학교 의과대학 병리학교실
이영하 ( Lee Young-Ha )
경북대학교 의과대학 외과학교실
김정완 ( Kim Jung-Wan )
경북대학교 치과대학 구강미생물학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
KMID : 0357920020360020100
Abstract
Background: Germline mutations in the breast cancer-associated genes BRCA1 and BRCA2 confer susceptibility and a lifetime risk of breast cancer. Several morphological and clinical features have been attributed to hereditary tumors. However, in sporadic breast cancer, the interrelationshp between the loss of heterozygosity (LOH) of these loci and clinical features remains to be fuly elucidated.
Methods: Microdissected paraffin-embedded tissue blocks of 48 cases of surgicall resected breast carcinoma were investigated to identify the LOH of BRCA1 and BRCA2 using microsatellite markers.
Results: Of 48 cases, 22(45.9%) exhibited LOH at BRCA1 locus while in 29 out of 48 (60.4%) cases LOH was observed for the BRCA2 region. There was no signicificant correlation between LOH was BECA1/2 and the patients’s age, tumor size, histologic grade or lymph node metastasis. When comparing the frequency of LOH with the expression of several prognostic factors, such as p53, c-erb B2 protein, estrogen and progesterone receptor using immunohistochmeical stain, there was only correlatio with LOH at BRCA2 and the progesterone receptor.
Conclusions: Our results suggest that allelic deletion play a role to the development of sporadic breast cancers.
키워드
Mammary Neoplasms;Loss of Heterozygosity-Genes;BRCA1-Genes;BRCA2
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