암육종과 악성 혼합상피종양에서의 클론형성능에 대한 연구
Clonality Study in Carcinosarcomas and Malignant Mixed Epithelial Tumors
박은정, 최유덕, 남종희, 이민철, 박창수, 정상우, 최인선, 김경희, 최찬,
소속 상세정보
박은정 ( Park Eun-Jung )
전남대학교 의과대학 병리학교실
최유덕 ( Choi Yoo-Duk )
전남대학교 의과대학 병리학교실
남종희 ( Nam Jong-Hee )
전남대학교 의과대학 병리학교실
이민철 ( Lee Min-Cheol )
전남대학교 의과대학 병리학교실
박창수 ( Park Chang-Soo )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
최인선 ( Choi In-Seon )
전남대학교 의과대학 병리학교실
김경희 ( Kim Kyung-Hee )
전남대학교 의과대학 병리학교실
최찬 ( Choi Chan )
전남대학교 의과대학 병리학교실
KMID : 0357920020360040205
Abstract
Background: Tumors are usually considered to be clonal progeny of single transformed cells. Carcinosarcomas and malignant mixed epithelial tumors are examples where controversies exist regarding the singularity or multiplicity of their cell of origin.
Methods: The authors examined the clonality of carcinosarcomas (7 cases) and malignant mixed epithelial tumor (5 cases) in female patients by X-chromosome inactivation as a marker. Each component of the tumors were picked up by the laser capture microscope. The polymorphic exon 1 CAG trinucleotide repeat in the X-linked human androgen receptor (HUMARA) gene was amplified by a polymerase chain reaction before and after treatment of the methylation-sensitive endonuclease HpaII.
Results: Eleven cases were informative for clonality determination. Six out of seven carcinosarcomas and three out of four malignant mixed epithelial tumors revealed the same patterns of X-chromosome inactivation, which suggests that they are monoclonal. In contrast, the patterns of X-chromosome inactivation were different between the two tumor components in each cases of carcinosarcoma and malignant mixed epithelial tumor, indicating that they are of polyclonal origin.
Conclusion: These observations show that although most of carcinosarcomas and malignant mixed epithelial tumors are of monoclonal origin, some of them are of polyclonal origin. This finding suggests that these tumors are genuinely polyclonal, and that they originated in the neoplastic transformation of more than one somatic cells.
키워드
Mixed tumor;Malignant;Carcinosarcoma;Clone Cells;X Chromosome
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