백서 간의 재관류 손상에서 단기간 허혈전처치의 효과 : Interleukin-1α 와 Nuclear Factor- ΚB 발현
The Effect of Ischemic Preconditioning in Rat Liver: The Expression of Interleukin-1α and Nuclear Factor-ΚB
금윤섭, 이수경, 김선주, 곽은경, 박지영, 박태인, 배한익, 손윤경, 서인수,
소속 상세정보
금윤섭 ( Kum Yoon-Seup )
경북대학교 의과대학 병리학교실
이수경 ( Lee Soo-Kyoung )
경북대학교 의과대학 병리학교실
김선주 ( Kim Sun-Joo )
경북대학교 의과대학 병리학교실
곽은경 ( Kwak Eun-Kyoung )
경북대학교 의과대학 병리학교실
박지영 ( Park Ji-Young )
경북대학교 의과대학 병리학교실
박태인 ( Park Tae-In )
경북대학교 의과대학 병리학교실
배한익 ( Bae Han-Ik )
경북대학교 의과대학 병리학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
서인수 ( Suh In-Soo )
경북대학교 의과대학 병리학교실
KMID : 0357920020360040238
Abstract
Background: A short period of ischemia and reperfusion, called ischemic preconditioning, protects various tissues against subsequent sustained ischemic insult. Apoptosis of hepatocytes and sinusoidal endothelial cells are a critical mechanisms of injury in the ischemic liver. Because nuclear factor-B (NF-B) has a significant role in the cell survival, we hypothesized that ischemic preconditioning protects by inhibition of apoptosis through the expression of NF-B, induced by interleukin-1 (IL-1), which is known for enhancement of its transcription and activation.
Methods: We induced ischemia and reperfusion on rat liver, and performed in situ terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labelling assay and polymerase chain reaction for IL-1 mRNA and NF-B mRNA.
Results: Apoptosis of hepatocytes and sinusoidal endothelial cells, assessed by in situ TUNEL assay, was significantly reduced with preconditioning. The expression of IL-1 mRNA and NF-B mRNA are seen on discrete monoclonal bands around 344 and 356 base pairs, in comparison with normal rat liver, but, there was no significant difference between the ischemia-reperfusion group and the preconditioning group.
Conclusion: We suggest that ischemic preconditioning confers dramatic protection against prolonged ischemia via inhibition of apotosis through the expression of IL-1 inducing NF-B and its activation. However, we need further study in the activity of NF-B, such as nucleotide shift assay, because the activity of NF-B is regulated by binding of the inhibitory protein, IB.
키워드
Reperfusion Injury;Ischemia;NF-Kappa B;Interleukin-1
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