어린이 위 점액층 내의 Helicobacter pylori 검출
Detection of Helicobacter pylori in the Gastric Mucous Layer in Pediatric Patients
김유경, 이종실, 김활웅, 이정희, 윤희상, 고경혁,
소속 상세정보
김유경 ( Kim Yu-Kyung )
경상대학교 의과대학 병리학교실
이종실 ( Lee Jong-Sil )
경상대학교 의과대학 병리학교실
김활웅 ( Kim Hwal-Woong )
경상대학교 의과대학 병리학교실
이정희 ( Lee Jeong-Hee )
경상대학교 의과대학 병리학교실
윤희상 ( Youn Hee-Shang )
경상대학교 의과대학 소아과학교실
고경혁 ( Ko Gyung-Hyuck )
경상대학교 의과대학 병리학교실
KMID : 0357920020360050292
Abstract
Background: Helicobacter pylori is present mainly in the gastric mucous layer. However, the mucous layer, along with the bacteria, is lost during conventional tissue processing in which formalin is used for fixation. The purpose of this study is to ascertain - if the mucous layer is preserved by using Carnoy solution as a fixative - whether the detection rate of H. pylori is increased in pediatric patients.
Methods: Five pieces of gastric mucosal tissue were obtained from the gastric antrum and the body of one hundred pediatric patients. One of the specimens was fixed with formalin. Another specimen was fixed with Carnoy solution. The tissue sections were stained with hematoxylin-eosin and immunohistochemically stained for H. pylori. For reference, a rapid urease test was performed on the remaining three specimens.
Results: In the formalin-fixed tissue, the detection rate of H. pylori was 13% in the gastric antrum and 12% in the body (overall 16%). In the Carnoy solution-fixed tissue, the mucous layer was preserved and the detection rate of H. pylori was 23% in the antrum and 27% in the body (overall 28%). The positive rate of the rapid urease test was 26% in the antrum and 28% in the body (overall 29%).
Conclusion: When the number of H. pylori is small in the gastric mucosa, the bacteria may not be detected by conventional histologic methods. In that case, the detection rate of H. pylori may be increased by using Carnoy solution, rather than formalin, as a tissue fixative.
키워드
Helicobacter pylori;Gastric Mucosa;Fixatives
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