World Health Organization/International Society of Urological Pathology 등급체계에 따른 방광 요로상피암종의 증식표지자로서 Ki-67 발현 및 세포자멸사와 비교한 DNA topoisomerase II-α의 발현 양상
Expression of DNA Topoisomerase II-α as a Proliferating Marker in Urothelial Carcinoma of Urinary Bladder based on World Health Organization/International Society of Urological Pathology Consensus Classification: A Correlation with Expression of Ki-67
이태진, 이동기, 박언섭, 유재형,
소속 상세정보
이태진 ( Lee Tae-Jin )
중앙대학교 의과대학 병리학교실
이동기 ( Lee Dong-Ki )
중앙대학교 의과대학 병리학교실
박언섭 ( Park Eon-Sub )
중앙대학교 의과대학 병리학교실
유재형 ( Yoo Jae-Hyung )
중앙대학교 의과대학 병리학교실
KMID : 0357920020360050305
Abstract
Background: DNA topoisomerase II-alpha is linked with active cell proliferation in mammalian cells. The aim of this study was to examine the relationship between the expression of DNA topoisomerase II-alpha as a proliferating marker, and the expression of Ki-67 and apoptosis in urothelial carcinoma of urinary bladder based on World Health Organization/International Society of Urological Pathology (WHO/ISUP) consensus classification.
Methods: 73 urothelial carcinomas of the urinary bladder after transurethral resection and 25 carcinomas after radical cystectomy were investigated for histologic grading based on WHO and WHO/ISUP consensus classification. Formalin fixed, paraffin embedded tissue of 98 specimens from 73 patients were immunohistochemically stained for DNA topoisomerase II-alpha and Ki-67, and in situ TdT-mediated dUTP-biotin nick end labeling method for evaluation of apoptotic cells was performed. For each case, a DNA topoisomerase II-alpha, Ki-67, and apoptotic indices were determined.
Results: The histologic grades of 73 cases based on the WHO grading system were 21.9% (16 cases) in grade 1, 65.8% (48 cases) in grade 2, and 12.3% (9 cases). 5.5% (4 cases) of papillary neoplasm of low malignant potential, 47.9% (35 cases) of urothelial carcinoma of low grade, and 46.6% (34 cases) in urothelial carcinoma of high grade were reclassified using the WHO/ISUP consensus classification. Histologic grades based on two grading systems were correlated to invasion and stage (p<0.05). DNA topoisomerase II-alpha, Ki-67, and apoptotic indices were correlated to histologic grades based on two grading system and invasion. Also, the correlation of DNA topoisomerase II-alpha and Ki-67 indices, and DNA topoisomerase II-alpha and apoptotic indices were significant, respectively.
Conclusion: DNA topoisomerase II-alpha appears to be an useful marker for assessing the proliferation potential of urothelial carcinoma of in the urinary bladder.
키워드
Carcinoma;Transitional Cell;DNA Topoisomerase;Type ll;Ki-67 Antigen;Apoptosis
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