진행위암에서 Nitric Oxide Synthase 아형의 발현에 관한 연구
Expression of Nitric Oxide Synthase Isotypes in Advanced Gastric Carcinoma
권경미, 주영채, 황태숙,
소속 상세정보
권경미 ( Kwon Kyong-Mee )
인하대학교 의과대학 병리학교실
주영채 ( Chu Young-Chae )
인하대학교 의과대학 병리학교실
황태숙 ( Hwang Tae-Sook )
인하대학교 의과대학 병리학교실
KMID : 0357920020360060374
Abstract
Background: Increased expression of nitric oxide synthase (NOS) isotypes is present in human tumor cell lines and solid tumor tissues. Hypoxia upregulates NOS expression, and nitric oxide (NO) induces mitogenesis among endothelial cells. NO has been known to induce vascular endothelial growth factor (VEGF) expression in carcinoma cells and to induce neovascularization in tumors.
Methods: The expression and cellular localization of 3 isotypes of NOS was detected by immunohistochemistry in 73 advanced gastric carcinoma tissues along with adjacent normal gastric mucosa; and the relationship to known clinicopathologic parameters, microvascular density, and VEGF expression was analysed.
Results: Forty-four (60.3%), 56 (76.7%), and 52 (71.2%) of the 73 cases revealed eNOS, nNOS, and iNOS expression, respectively. Intestinal type adenocarcinomas tended to have higher activity of eNOS (p=0.000) and nNOS (p=0.001) activities than did the diffuse type adenocarcinomas. All isotypes of NOS (eNOS, p=0.001; nNOS, p=0.005; iNOS, p=0.044) tended to be highly expressed when the tumor was differentiated. There was no significant relationship between any of the 3 NOS isotypes and microvascular density, whereas VEGF was closely related with microvascular density (p=0.000). The expression of VEGF was not related to with any of the NOS isotype expressions.
Conclusion: From the above results, we speculated that NO may be implicated in the early stage of the gastric carcinogenesis rather than the growth and progression stages, and NO does not appear to affect angiogenesis or VEGF expression in the advanced gastric carcinoma.
키워드
Stomach Neoplasms;Angiogenesis Factor;Nitric Oxide Synthase
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