Lipopolysaccharide/Interferon-gamma에 의한 C6 Glioma 세포주의 Nitric Oxide 생성 양상과 Dexamethasone의 억제효과
Lipopolysaccharide/Interferon-gamma Induced Nitric Oxide Production in C6 Glioma Cells: Modulation by Dexamethasone
신종헌, 강구성, 김지연, 김선주, 박지영, 곽은경, 손윤경,
소속 상세정보
신종헌 ( Shin Jong-Heun )
경북대학교 의과대학 병리학교실
강구성 ( Kang Ku-Seong )
경북대학교 의과대학 병리학교실
김지연 ( Kim Ji-Yeoun )
경북대학교 의과대학 병리학교실
김선주 ( Kim Sun-Joo )
경북대학교 의과대학 병리학교실
박지영 ( Park Ji-Young )
경북대학교 의과대학 병리학교실
곽은경 ( Kwak Eun-Kyoung )
경북대학교 의과대학 병리학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
KMID : 0357920020360060406
Abstract
Background: Glial cell-derived nitric oxide (NO), and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to see the production of NO in lipopolysaccharide (LPS)/interferon-gamma (IFN-)-treated C6 glioma cells and the effect of dexamethasone on NO production and apoptosis of LPS/IFN--treated C6 glioma cells.
Methods: The apoptosis of LPS/IFN- treated C6 glioma cell was examined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method and the production of NO in culture medium was measured. The expression of iNOS mRNA was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The effect of the N-monomethyl L-arginine (NMMA) and dexamethasone on the apoptosis and NO production was also examined.
Results: Inducible nitric oxide synthase (iNOS) mRNA and NO production were markedly increased in LPS/IFN--treated C6 glioma cells. The expression of iNOS mRNA arose at 3 hours, peaked at 12 hours, and declined 24 hours after LPS/IFN--treatment. Accumulation of NO derivatives in the culture media was increased at least upto 48 hours after LPS/IFN-. The induction of iNOS expression and NO production in LPS/IFN--treated C6 cells was correlated with apoptotic cell death judged by TUNEL staining. After treatment of NMMA, one of the NOS inhibitors, NO production and apoptosis were markedly decreased. Dexamehasone treatment suppressed the NO production by concentration depenedent manner.
Conclusion: From the above results it is concluded that the LPS/IFN- induced apoptosis of C6 cells is mediated by iNOS-derived NO and NO production and apoptosis was suppressed by dexamethasone.
키워드
Nitric Oxide Synthase;Nitric Oxide;Lipopolysaccharides Dexamethasone;Apoptosis
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