유방암에서 색소원제자리부합화에 의한 HER-2/neu 종양유전자 증폭과 Topoisomerase II-α의 면역조직화학적 발현
HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization and Immunohistochemical Expression of Topoisomerase II-α in the Breast Cancer
이태진, 오형균, 권귀영, 김미경, 박언섭, 유재형,
소속 상세정보
이태진 ( Lee Tae-Jin )
중앙대학교 의과대학 병리학교실
오형균 ( Oh Hyung-Goon )
중앙대학교 의과대학 병리학교실
권귀영 ( Kwon Gui-Young )
중앙대학교 의과대학 병리학교실
김미경 ( Kim Mi-Kyung )
중앙대학교 의과대학 병리학교실
박언섭 ( Park Eon-Sub )
중앙대학교 의과대학 병리학교실
유재형 ( Yoo Jae-Hyung )
중앙대학교 의과대학 병리학교실
KMID : 0357920030370010026
Abstract
Background: Amplifications of the HER-2/neu oncogene and the Topoisomerase II-α gene are important determiners of the response to chemotherapy in the breast cancer. For detecting HER-2/neu amplification, fluorescent in situ hybridization and immunohistochemistry are currently regarded as standard methods. Chromogenic in situ hybridization (CISH) is investigated as a new modification of in situ hybridization. The purpose of this study is to compare the efficacy of CISH and immunohistochemistry (IHC) in detecting HER-2/neu oncogene amplification and to investigate the prognostic significance of the HER-2/neu oncogene and the Topoisomerase II-α gene in breast cancer.
Methods: Using CISH and IHC the amplifications and protein expressions of the HER-2/neu oncogene were studied on paraffin sections of 43 infiltrating duct carcinomas. The expression of the Topoisomerase II-α gene was studied immunohistochemically.
Results: Of the 43 infiltrating duct carcinomas, amplifications of the HER-2/neu oncogene by CISH were observed in 8 cases (18.6%), and the HER-2/neu protein was deemed overexpressed by IHC in 9 cases (20.9%). The amplifications of the HER-2/neu oncogene showed a statistically significant correlation with tumor size, histological grade, and the Topoisomerase II-α index. The Topoisomerase II-α index showed a statistically significant correlation with tumor size, lymph node status, stage, histologic grade, and estrogen receptor status.
Conclusion: CISH is a useful alternative for determining HER-2/neu amplification, especially for confirming the immunohistochemical staining results. HER-2/neu amplification and the Topoisomerase II-α gene index may be prognostic factors of breast cancer.
키워드
Mammary Neoplasms;In situ Hybridization;HER-2/neu-DNA Topoisomerase type II-α
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