Expression of Transforming Growth Factor-beta and Its Receptors during Acetic Acid-induced Duodenal Ulcer Healing in Rats
장규윤, Yoon Han-Sang, 유기훈, 정명자, 이동근, 강명재,
소속 상세정보
장규윤 ( Jang Kyu-Yun )
전북대학교 의과대학 병리학교실
( Yoon Han-Sang )
전북대학교 의과대학 병리학교실
유기훈 ( Yu Ki-Hoon )
전북대학교 의과대학 병리학교실
정명자 ( Chung Myung-Ja )
전북대학교 의과대학 병리학교실
이동근 ( Lee Dong-Geun )
전북대학교 의과대학 병리학교실
강명재 ( Kang Myoung-Jae )
전북대학교 의과대학 병리학교실
KMID : 0357920030370020108
Abstract
Background: Many works have been written about the transforming growth factor-beta1 (TGF-beta1) which is closely associated with fibrosis in the inflammatory conditions of different organs. TGF-beta1 exerts its biological effects by interacting with specific cell surface receptors, namely, transforming growth factor-beta receptor type I and II (TGFbetaRI and TGFbetaRII).
Methods: To investigate the temporal expressions and localizations of TGF-beta1, TGFbetaRI, and TGFbetaRII in acetic acid-induced duodenal ulcerated tissues, we performed in situ hybridization and immunohistochemical techniques.
Results: Under in situ hybridization, TGF-beta1, TGFbetaRI, and TGFbetaRII mRNA signals increased in the experimental groups (1, 3, 7, and 14 day groups) compared to those of the control group. The signals on day 14 decreased slightly compared to those of days 1, 3, and 7, but they were higher than those of the control group. Under immunohistochemical study, TGF-beta1, TGFbetaRI, and TGFbetaRII were localized in the mucosal epithelial cells and in the macrophages, vascular endothelial cells, and fibroblasts of the lamina propria and granulation tissue. As in the case of the in situ hybridization, it revealed that the expression of three proteins increased in the experimental groups compared to that of the control group. The expression on day 14 decreased compared to those of days 1, 3, and 7, but it was more intense than that of the control group.
Conclusion: This study suggests that TGF-beta1, TGFbetaRI, and TGFbetaRII contribute to the early stage healing of duodenal ulcer.
키워드
Transforming Growth Factor beta-Receptor;Transforming Growth Factor beta Ulcer;In Situ Hybridization;Immunohistochemistry
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