NK/T 세포 림프종과 말초 T-세포 림프종에 잠복 감염된 Epstein-Barr virus의 Major Latent Promoter C의 메틸화 분석
Methylation Status of Epstein-Barr Virus Major Latent Promoter C in NK/T-cell Lymphoma and Peripheral T-cell Lymphoma
김지은, 김영아, 박성신, 전윤경, 이승숙, 김철우,
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김지은 ( Kim Ji-Eun )
서울시립보라매병원 병리과
김영아 ( Kim Young-A )
서울시립보라매병원 병리과
박성신 ( Park Sung-Shin )
서울대학교 의과대학 병리학교실
전윤경 ( Jeon Yoon-Kyung )
서울대학교 의과대학 병리학교실
이승숙 ( Lee Seung-Sook )
원자력병원 병리학과
김철우 ( Kim Chul-Woo )
서울대학교 의과대학 병리학교실
KMID : 0357920030370030174
Abstract
Background: Both the Natural killer/T-cell lymphoma (NKTL) and the peripheral T-cell lymphoma (PTCL) are relatively prevalent in the Asian population, and they are strongly associated with the Epstein-Barr virus (EBV). These two diseases have several common pathologic features, but show somewhat different clinical presentations. The critical point in terms of differentiating of these disease groups might be the impact of EBV in pathogenesis, and the variable gene expression of EBV regulated by a major latent C promoter (Cp).
Methods: We investigated 43 cases of NKTL and 30 cases of PTCL to evaluate EBV associated characteristics. EBV in situ hybridization was performed in all of the submitted cases. In the EBV positive cases, the methylation status of Cp which drives the expression of immunodominant viral nuclear protein, was examined by sensitive methylation specific PCR using paraffin embedded tissue.
Results: EBV was found in 70% (30/43) of NKTL and 43% (13/30) of PTCL. Nasal and gastrointestinal lymphomas were predominantly NKTL. All of the successfully amplified cases of EBV positive NKTL and PTCL were of methylated Cp status.
Conclusion: The detection rate of EBV is high in NKTL, especially in the nasal area. The constantly methylated EBV Cp reflects the major role of Cp in regulating the EBV latency pattern and in helping EBV to avoid host immune system in both NKTL and PTCL.
키워드
Herpesvirus 4;Human-Lymphoma;T-cell-Promoter regions;Methylation
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