신세포암종에서 cDNA Microarray를 이용한 유전자 발현에 관한 연구 - Decorin 발현의 임상병리학적 분석
Analysis of Gene Expression in Renal Cell Carcinomas Using cDNA Microarray - Reduced Expression of Decorin in Renal Cell Carcinomas
이진숙, 서강석, 최경운, 김지연, 박도윤,
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이진숙 ( Lee Jin-Sook )
부산대학교 의과대학 병리학교실
서강석 ( Suh Kang-Suek )
부산대학교 의과대학 병리학교실
최경운 ( Choi Kyung-Un )
부산대학교 의과대학 병리학교실
김지연 ( Kim Jee-Yeon )
부산대학교 의과대학 병리학교실
박도윤 ( Park Do-Youn )
부산대학교 의과대학 병리학교실
KMID : 0357920030370040232
Abstract
Background: Identification of the genes expressed differentially in renal cell carcinoma (RCC) but not in the non-cancerous kidney is important for understanding the molecular basis of renal cell carcinoma and for defining possible prognostic value and therapeutic intervention. We investigated the changes in gene expression accompanying the development and progression of kidney cancer by cDNA microarrays.
Methods: To identify molecular alterations in renal cell carcinoma, we measured expression profiles for paired neoplastic and noncancerous kidney samples from an individual by means of a cDNA microarray representing 7,500 genes. Of the differentially expressed genes, we assessed the decorin gene at the protein level using immunohistochemistry.
Results: The 60 genes were noted to have more than a fivefold change in expression (either increased or decreased) in RCC compared to the non-cancerous kidney. The changed genes are those associated with signal transduction, metabolizing enzymes, the cytoskeleton, cell adhesion, cell cycle control, modulation of transcription, the tumor suppressor gene and tumor antigens. Under immunohistochemistry, the expression of decorin was significantly decreased in the tumor than in the non-cancerous kidney. The expression rate of decorin was not associated with the patient’s sex, age, histologic type, Fuhrmann nuclear grade and T stage.
Conclusions: The author predicted that these gene expression profiling experiments will lead to improvements in the basic understanding of the renal tumor pathogenesis and will promote the discovery of novel molecular markers for renal tumor diagnosis and therapy.
키워드
Renal cell carcinoma;Microarray;Decorin
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