위장관 간질종양에서 c-kit 유전자 돌연변이 및 면역조직화학적 발현
c-kit Mutation and Immunohistochemical Expression in Gastrointestinal Stromal Tumors
강동욱, 김주헌, 김동훈, 김경희, 박미자, 강대영,
소속 상세정보
강동욱 ( Kang Dong-Wook )
을지대학교 의과대학 병리학교실
김주헌 ( Kim Ju-Hun )
을지대학교 의과대학 병리학교실
김동훈 ( Kim Dong-Hun )
을지대학교 의과대학 병리학교실
김경희 ( Kim Kyung-Hee )
을지대학교 의과대학 병리학교실
박미자 ( Park Mee-Ja )
을지대학교 의과대학 병리학교실
강대영 ( Kang Dae-Young )
충남대학교 의과대학 병리학교실
KMID : 0357920030370040246
Abstract
Background: Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract. The aim of this study is to investigate the correlation among the clinicopathologic features, presence of c-kit mutation, and immunohistochemical expression of c-kit in 61 cases of GISTs.
Methods: We divided the GISTs into three groups as benign, borderlines and malignant, according to histologic grade. Exon 11 of the c-kit was amplified by PCR and sequenced. We performed immunohistochemical study for CD117, CD34, vimentin, SMA, desmin, and S-100 protein.
Results: Twenty-one cases were diagnosed as benign GISTs, 14 cases as borderline GISTs, and 26 cases as malignant GISTs. The shape, atypia, cellularity, and necrosis showed good correlations with the histologic grades of the GISTs. Mutations of exon 11 of the c-kit were detected in 3 benign GISTs, 4 borderline GISTs, and 13(%) malignant GISTs. Sequence analysis confirmed the deletion mutation (n=16) and the single base pair mutation (n=4). The immunohistochemical stainings showed myogenic differentiation (n=20), neurogenic differentiation (n=15), and neither myogenic or neurogenic differentiation (n=34).
Conclusions: The GIST is the primitive mesenchymal tumor capable of divergent differentiation, and the mutation of the c-kit is a good parameter for the malignant GIST.
키워드
Gastrointestinal Stromal Tumor;Protooncogene Protein c-kit;Immunohistochemistry
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