N-butyl-N-[4-hydroxybutyl]nitrosamine에 의한 흰쥐 방광암 발생 과정에서 세포주기 관련인자 단백[Cyclin D1, A, E, and B]의 발현
Expression of Cyclins (D1, A, E, and B1) in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced Rat Bladder Carcinogenesis
권귀영, 박언섭, 봉성근, 이태진, 김미경, 유재형, 송계용,
소속 상세정보
권귀영 ( Kwon Gui-Young )
중앙대학교 의과대학 병리학교실
박언섭 ( Park Eon-Sub )
중앙대학교 의과대학 병리학교실
봉성근 ( Bong Sung-Geun )
중앙대학교 의과대학 병리학교실
이태진 ( Lee Tae-Jin )
중앙대학교 의과대학 병리학교실
김미경 ( Kim Mi-Kyung )
중앙대학교 의과대학 병리학교실
유재형 ( Yoo Jae-Hyung )
중앙대학교 의과대학 병리학교실
송계용 ( Song Kye-Yong )
중앙대학교 의과대학 병리학교실
KMID : 0357920030370040255
Abstract
Background: Cell cycle deregulation plays a major role in chemical multistage carcinogenesis. Therefore, the evaluation of cell cycle proteins is important.
Methods: In order to induce carcinogenesis in the rat urinary bladder, 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) was administered to male Sprague-Dawley rats for 30 weeks. Expression of cyclin D1, A, E, and B1 were examined by immunohistochemical stainings.
Results: Urothelial cell hyperplasia appeared at 5 weeks, followed by papilloma at 10 weeks. Superficial carcinoma was observed at 20 weeks, and invasive carcinoma developed in 40% (4/10) of the rats at 30 weeks. Expressions of cyclin D1 and A increased sequentially from normal mucosa through hyperplasia, papilloma, and carcinoma (p<0.01). Expressions of cyclin D1, B1, and cyclin E were higher in invasive carcinomas than in superficial carcinomas (p<0.01). In contrast, there was no significant difference in the expression of cyclin B1 between hyperplasia, papilloma, and superficial carcinoma.
Conclusions: The present results indicate the important roles of cyclin D1 and A in the development of BBN-induced urothelial carcinoma of rats. Aberrant expression of cyclin B1 and E may contribute to the progression from superficial to invasive bladder cancer rather than tumorigenesis.
키워드
Cyclins;Carcinogenesis;N-butyl-(4-hydroxybutyl)nitrosamine;Rat;Urinary bladder
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