만성 육아종성 염증에서 파라핀 포매 조질을 이용한 결핵균 PCR 검사외 Ziehl-Neelsen 염색의 비교 분석
Comparison of Ziehl-Neelsen Stain and TB-PCR on Detection of Mycoboctedum tuberculosis in Formalin-fixed, Paraffin-embedded Tissues of Chronic Granulomatous Inflammation
조민선, 성순희, 한운섭, 이시내,
소속 상세정보
조민선 ( Cho Min-Sun )
이화여자대학교 의과대학 병리학교실
성순희 ( Sung Sun-Hee )
이화여자대학교 의과대학 병리학교실
한운섭 ( Han Woon-Sup )
이화여자대학교 의과대학 병리학교실
이시내 ( Lee Shi-Nae )
이화여자대학교 의과대학 병리학교실
KMID : 0357920030370060379
Abstract
Background: TB-PCR is a faster and more sensitive method to detect mycobacterium than acid-fast bacilli (AFB) stain, which is laborious and time consuming. We compared the sensitivity and specificity of AFB stain and TB-PCR and examined the possibility of TB-PCR as a confirmative test without AFB stain in the diagnosis of tuberculosis.
Methods: We performed Ziehl-Neelsen stain and nested PCR using a commercially available TB-PCR kit amplifying IS61 10 sequence in 81 cases of paraffin-embedded tissues diagnosed as chronic granulomatous inflammation. In addition, we evaluated the morphology of granuloma and the presence of caseation necrosis.
Resuits: Of the 81 cases studied, 22(27.2%) and 40(49.4%) were positive for AFB stain and TB-PCR, respectively. Of 49 cases accompanying caseation necrosis, 19(38.8%)were AFB stain positive and 37(75.5%) were TB-PCR positive; a result that is comparable with that of other reports. Of the 22 AFB-positive cases, 2 were TB-PCR negative.
Conclusion: TB-PCR is very helpful for the diagnosis of tuberculosis in routinely processed, formalin-fixed, paraffin-embedded tissue samples. Nevertheless, AFB stain should continue to be performed at the same time.
키워드
Chronic Granulomatous Diseases;Mycobacterium tuberculosis;Stains;PCR
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