Clonal Analysis of Neurofibroma by PCR Amplification of HUMARA Gene
이제혁, 한승상, 오현식, 최유덕, 김현중, 이경화, 남종희, 최찬, 정상우,
소속 상세정보
이제혁 ( Lee Jae-Hyuk )
전남대학교 의과대학 병리학교실
한승상 ( Han Seung-Sang )
전남대학교 의과대학 재활의학교실
오현식 ( Oh Hyun-Sik )
전남대학교 의과대학 병리학교실
최유덕 ( Choi Yoo-Duk )
전남대학교 의과대학 병리학교실
김현중 ( Kim Hyun-Joong )
전남대학교 의과대학 병리학교실
이경화 ( Lee Kyung-Hwa )
전남대학교 의과대학 병리학교실
남종희 ( Nam Jong-Hee )
전남대학교 의과대학 병리학교실
최찬 ( Choi Chan )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
KMID : 0357920030370060421
Abstract
Background: While neurofibromas have generally been regarded as polyclonal hyperplastic lesions, it remains unclear whether the tumor is a true neoplasm or a hyperplastic lesion.
Methods: Determination of clonality by chromosome inactivation pattern was investigated in twenty-one cases of neurofibroma employing enzyme digestion and PCR of the HUMARA gene. The histological, immunohistochemical, and ultrastructural characteristics of the tumors were also examined.
Results: Immunohistochemicaly, most of the tumor cells showed vimentin and S-100 protein positivity. Axons were demonstrated by neurofilament protein positivity and were seen mainly at the periphery and rarely in the central portion of the tumor. Ultrastructurally, the tumors were composed of a variety of cell types: perineurial cells, Schwann cells, fibroblasts, and axons. chromosome inactivation analysis was completed on thirteen out of fifteen cases in which DNA was successfully extracted. Of thirteen neurofibromas that were heterozygous at the HUMARA loci, eleven showed a polyclonal pattem. The remaining two cases were considered as indeterminate for clonality because of unequal band intensity and failure to obtain the normal control DNA.
Concluslon: The results from this study suggest that neurofibromas are polyclonal in origin and might be a neoplastic lesion comprisisng non-neoplastic cells among constituent components.
키워드
Neurofibroma;Clonality;HUMARA;Immunohistochemistry;Ultrastructure
원문 및 링크아웃 정보
등재저널 정보