Ginsenoside Rb1이 C6 Glioma 세포주의 Nitric Oxide 생성과 세포자멸사에 미치는 효과
The Effects of Ginsenoside Rb1 on the Apoptosis and the Production of Nitric Oxide in Rat C6 Glioma Cells
박주현, 이윤희, 강구성, 이수경, 김선주, 박지영, 곽은경, 손윤경,
소속 상세정보
박주현 ( Park Ju-Hyun )
경북대학교 의과대학 병리학교실
이윤희 ( Lee Yoon-Hee )
경북대학교 의과대학 병리학교실
강구성 ( Kang Ku-Seong )
경북대학교 의과대학 병리학교실
이수경 ( Lee Soo-Kyoung )
경북대학교 의과대학 병리학교실
김선주 ( Kim Sun-Joo )
경북대학교 의과대학 병리학교실
박지영 ( Park Ji-Young )
경북대학교 의과대학 병리학교실
곽은경 ( Kwak Eun-Kyoung )
경북대학교 치과대학 구강병리학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
KMID : 0357920040380010001
Abstract
Background: Ginsenosides, the extract of Panax ginseng, exert various pharmacological effects such as anticancer activity by the mechanism that is not yet defined. In this study, we proposed that the anticancer effect of ginsenoside Rb1 is related to tumor cell apoptosis and ginsenoside Rb1 induces the tumor cell apoptosis via the nitric oxide (NO) production.
Methods: Rat C6 glioma cells were activated by treating with lipopolysaccharide (LPS), interferon (IFN)-γ, and tumor necrosis factor (TNF)-α on the culture medium to investigate the effects of ginsenoside Rb1.
Results: Compared with C6 glioma cells treated with LPS/IFN-γ/TNF-α, C6 glioma cells treated with LPS/IFN-γ/TNF-α/ginsenoside Rb1 showed marked increase in the NO production and apoptosis. Ginsenoside Rb1 induces the NO production in C6 glioma cells in dose-dependent manner. When C6 glioma cells treated with LPS/IFN-γ/TNF-α/ginsenoside Rb1 were incubated with the specific inhibitor of iNOS, S-Methyl-2-thiopseudourea-sulfate (SMT), both NO production and apoptosis in C6 glioma cells was significantly decreased. Ginsenoside Rb1 induced the expression of iNOS mRNA and iNOS protein in C6 glioma cells.
Conclusions: These results suggest that the induction of iNOS expression and subsequent NO production by ginsenoside Rb1 induces apoptosis in C6 glioma cells.
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