Genomic Imbalances in Ependymoma by Degenerate Oligonucleotide Primed PCR-Comparative Genomic Hybridization
박성혜, 김한성, 박선화, 김민경, 김기진, Suh Yoen-Lim,
소속 상세정보
박성혜 ( Park Sung-Hye )
서울대학교 의과대학 병리학교실
김한성 ( Kim Han-Seong )
인제대학교 의과대학 일산백병원 병리과
박선화 ( Park Sun-Hwa )
고려대학교 의과대학 해부학교실
김민경 ( Kim Min-Kyung )
인제대학교 의과대학 일산백병원 병리과
김기진 ( Kim Gi-Jin )
고려대학교 의과대학 해부학교실
( Suh Yoen-Lim )
성균관대학교 의과대학 삼성서울병원 병리과
KMID : 0357920040380030133
Abstract
Background: The most consistent chromosomal abnormality in ependymomas, is loss of 22q (17-75%) and gain of 1q (0-50%). However, significance of this abnormality is uncertain.
Methods: Genomic imbalances in 27 Korean ependymomas, including 21 low grade ependymomas, 4 anaplastic and 2 myxopapillary ependymomas, were analyzed by degenerate oligonucleotide primed-PCR-comparative genomic hybridization.
Results: Common gains were found in 17 (63%), 20q (59%), 9q34 (41%), 15q24-qter (33%), 11q13 (30%), 12q23 (26%), 7q23-qter (26%), 16q23-qter (30%), 19 (26%), and 1q32-qter (22%). DNA amplification was identified in 12 tumors (44%). Chromosomal loss was a less common occurrence in our study, but was found in 13q (26%), 6q (19%), and 3 (11%).
Conclusion: The recurrent gains or losses of the chromosomal regions which were identified in this study provide candidate regions that may be involved in the development and progression of ependymomas.
키워드
Ependymoma;Comparative Genomic Hydridization;Chromosomal aberration;PCR
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