쥐 해마의 Lipopolysaccharide 국소주입에 의한 Tumor Necrosis Factor-α, Interleukin-1β 및 Inducible Nitric Oxide Synthase의 발현
Expression of Tumor Necrosis Factor-α, Interleukin-1β and Inducible Nitric Oxide Synthase after Stereotaxic Injection of Lipopolysaccharide in Rat Hippocampus
오훈규, 박지영, 강구성, 김정완, 손윤경, 곽은경, 김지연,
소속 상세정보
오훈규 ( Oh Hoon-Kyu )
대구가톨릭대학교 의과대학 병리학교실
박지영 ( Park Ji-Young )
성균관대학교 의과대학 삼성제일병원 병리과
강구성 ( Kang Ku-Seong )
경북대학교 의과대학 병리학교실
김정완 ( Kim Jung-Wan )
경북대학교 치과대학 구강미생물학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
곽은경 ( Kwak Eun-Kyoung )
경북대학교 치과대학 구강병리학교실
김지연 ( Kim Ji-Yeon )
경북대학교 의과대학 병리학교실
KMID : 0357920040380030157
Abstract
Background:inducible nitric oxide synthase (iNOS) might be detectable in several pathologic conditions, and it is thought to play an important role in their pathophysiology. Tumor necrosis factor (TNF)-α and interleukin (IL)-1β are believed to be essential factors of iNOS induction of the brain.
mothods: After intrahippocampal stereotaxic injection of lipopoly-saccharide (LPS), the rat brains were removed at 6, 12 and 24 h. The rat brain tissues were examined to clarify the expression patterns of TNF-α, IL-1β and iNOS.
Results: The inflammatory cells which were stained with anti-TNF-α antibody, appeared in 6 h and increased for 24 h after LPS injection. The iNOS positive cells appeared after 12 h of LPS injection. A semiquantitative analysis of reverse transcription-polymerase chain reaction (RT-PCR) revealed that the TNF-α and IL-1β mRNA arose at 1 h, peaked at 6 h and then declined until 48 h after LPS injection. The iNOS mRNA arose after 6 h, peaked at 12 h, and declined until 48 h after LPS injection.
Conclusions: We conclude that the induction of inflammatory events by intrahippocampal injection of LPS activates TNF-α and IL-1β secretion, and this is followed by an induction of iNOS expression. TNF-α and IL-1β seem to be related with iNOS expression in brain inflammation.
키워드
Inducible Nitric Oxide Synthase;Tumor Necrosis Factor;Interleukins;Lipopoly;saccharides;Hippocampus
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