육종에서 p53, c-kit과 CD34의 발현에 대한 조직 미세배열을 이용한 연구
Tissue Microarray Analysis of the Expression of p53, c-kit and CD34 in Sarcomas
유진영, 강창석, 강석진, 박경신,
소속 상세정보
유진영 ( Yoo Jin-Young )
가톨릭대학교 의과대학 임상병리학교실
강창석 ( Kang Chang-Suk )
가톨릭대학교 의과대학 임상병리학교실
강석진 ( Kang Seok-Jin )
가톨릭대학교 의과대학 임상병리학교실
박경신 ( Park Gyeong-Sin )
가톨릭대학교 의과대학 임상병리학교실
KMID : 0357920040380040221
Abstract
Background: Our objectives in this study were to (1) evaluate the possible role of p53, c-kit and CD34 proteins in sarcomas and to determine their potential relationship; (2) use a tissue microarray to compare the immunohistochemical staining results on both the tissue microarrays and the corresponding whole tissue sections.
Methods: Whole sections from 85 sarcomas were studied for the immunohistochemical expression of p53, c-kit and CD34. Tissue microarrays consisting of triplicate 2 mm cores from the corresponding blocks were constructed and stained according to the same protocols as those used for the whole sections.
Results: On whole section analysis, p53 protein was expressed in 25 cases (29.4%). Expression of c-kit was observed in 31 specimens (36.5%), whereas CD34 expression was noted in 11 tumors (12.9%). The overall concordance between triplicates was 96% (217/226). The consensus score from the combined triplicates agreed with the results on the whole sections at 91.4% (233/255). The correlations between p53 and CD34, and between c-kit and CD34, were statistically significant (p=.028 and p=.010 respectively).
Conclusions: p53 and c-kit express relatively frequently in sarcomas. Tissue microarrays are an effective alternative to whole sections; however, the presence of triplicate punches seems to improve the yield but not the concordance of data.
키워드
Sarcoma;Tissue Microarray;p53;c-kit;CD34
원문 및 링크아웃 정보
등재저널 정보