내분비계 장애물질에 의한 고환 내 세포손상에서 아포토시스 관련 분자들의 변화
The Apoptotic Molecular Changes of Cellular Injury in Mouse Testis Induced by Endocrine Disrupting Chemicals
왕은휘, 이권행, 정은선, 강창석, 최영진, 양기화, 이진숙,
소속 상세정보
왕은휘 ( Wang Eun-Hui )
가톨릭대학교 의과대학 임상병리학교실
이권행 ( Lee Kweon-Haeng )
가톨릭대학교 의과대학 약리학교실
정은선 ( Jung Eun-Sun )
가톨릭대학교 의과대학 임상병리학교실
강창석 ( Kang Chang-Suk )
가톨릭대학교 의과대학 임상병리학교실
최영진 ( Choi Yeong-Jin )
가톨릭대학교 의과대학 임상병리학교실
양기화 ( Yang Ki-Hwa )
식품의약품안전청
이진숙 ( Lee Jin-Suk )
가톨릭대학교 의과대학 약리학교실
KMID : 0357920040380040228
Abstract
Background: Spermatogenesis is regulated by various cellular reactions, and especially cell proliferation and apoptosis.
Methods: We investigated the morphological changes and the apoptotic molecular changes in mouse testis induced by the endocrine disrupting chemicals. ICR mice were treated with bisphenol A (BPA), 2-bromopropane (2-BP) and diethylstilbesterol (DES). Histological examination and immunohistochemical staining, TUNNEL staining and RNAse protection assay were conducted.
Results: Testes treated with BPA showed normal spermatogenesis and the proliferation activity, and the density of the cells was similar with those in the control. 2-BP and DES groups, which showed a decrease of germ cells near the basal layer and degenerative changes. The proliferative activity identified by PCNA staining was significantly decreased in the 2-BP and DES groups (p<0.05). The apoptosis was significantly increased in the 2-BP group however, a significant decrease was noted in the BPA group (p<0.05). Among apoptosis-related molecules, the expression of Fas, Fas ligand, TRAIL, TNFp55 and caspase 1, 3, 6 and 8 were changed according to the change of the degree of apoptosis in all groups.
Conclusions: Endocrine disrupting chemicals induced cellular injury in mouse testis through the changes of proliferative activity and apoptosis which was regulated by a number of apoptosis-related molecules. This probably results in the abnormality of spermatogenesis in mouse testis.
키워드
Bisphenol A;2-Bromopropane;Diethylstilbesterol;Spermatogenesis;Testis
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