이질통증의 발생에 대한 Minocycline의 완화 효과: 흰쥐의 척수 뒤뿔내 CD11b, GFAP 및 c-Fos에 대한 면역조직화학 연구
Minocycline Attenuates the Development of Allodynia: An Immunohistochemical Study on CD11b, GFAP and c-Fos in the Spinal Dorsal Horn in SD Rat
강구, 강성식, 김성수, 전완주, 안영준, 최기용, 이민섭, 정일영,
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강구 ( Kang Gu )
강원대학교 의과대학 병리학교실
강성식 ( Kang Seong-Sik )
강원대학교 의과대학 마취통증의학교실
김성수 ( Kim Sung-Soo )
강원대학교 의과대학 약리학교실
전완주 ( Chun Wan-Joo )
강원대학교 의과대학 약리학교실
안영준 ( Ahn Young-Jun )
강원대학교 의과대학 병리학교실
최기용 ( Choi Ki-Young )
강원대학교 의과대학 병리학교실
이민섭 ( Lee Min-Sup )
강원대학교 의과대학 병리학교실
정일영 ( Cheong Il-Young )
강원대학교 의과대학 마취통증의학교실
KMID : 0357920040380050311
Abstract
Background: Minocycline, a semisynthetic second-generation tetracycline, is an antibiotic that has excellent ability to penetration into the CNS via the brain-blood barrier. Minocycline has emerged as a potent inhibitor of microglial activation, and it is an effective neuroprotective agent in experimental brain ischemia. Glial cell activation and proliferation are known to be associated with neuropathic pain in the peripheral nerve injuries.
Methods: The fifty percent threshold of withdrawal responses was measured in the hindpaws of SD rats following tight ligation of left fifth lumbar spinal nerve. Rats were sacrificed at 1, 3, 5, and 7 days and at 0.5, 1, 2, and 4 h post ligation (n=5/group/time point). Immunohistochemistry for GFAP, CD11b and c-Fos was done on the spinal cord at the level of the fifth lumbar nerve. Minocycline (45 mg/kg) and normal saline (300-400 L) were administered intraperitoneally, 1 day and 1 h before the operations, and every day postoperatively until the rats were sacrificed.
Results: Treatment with minocycline reduced allodynia and the expressions of CD11b at 5 days and c-Fos at 1 and 2 h post operation compared with the saline treatment (control).
Conclusions: It was thought that minocycline reduced the allodynia induced by tight ligation of the fifth lumbar spinal nerve in rats through the inhibition of microglial activation and c-Fos expression.
키워드
Neuropathy;Pain;Minocycline;Microglia
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