난소종양에서의 Melanoma Antigen Gene (MAGE) 와 Synovial Sarcoma on X chromosome (SSX)의 발현
Expression of Melanoma Antigen Gene (MAGE) and Synovial Sarcoma on X chromosome (SSX) in Ovarian Tumors
김영옥, 박종욱, 장희경, 박진경, 김광휘, 전창호, 김원,
소속 상세정보
김영옥 ( Kim Young-Ok )
고신대학교 의과대학 병리학교실
박종욱 ( Park Jong-Wook )
계명대학교 의과대학 면역학교실
장희경 ( Chang Hee-Kyung )
고신대학교 의과대학 병리학교실
박진경 ( Park Jean-Kyung )
고신대학교 의과대학 병리학교실
김광휘 ( Kim Kwang-Hui )
고신대학교 의과대학 병리학교실
전창호 ( Jeon Chang-Ho )
대구가톨릭대학교 의과대학 진단검사의학교실
김원 ( Kim Won )
경남정보대학 의무행정과
KMID : 0357920040380060372
Abstract
Background: Several cancer-testis antigen genes or gene families have been isolated to date, including Melanoma Antigen Gene (MAGE) and Synovial Sarcoma on X chromosome (SSX). This study attempted to investigate the possibility of immunotherapy for ovarian cancer and to explore the prevalence of the expression of MAGE and SSX.
Methods: The fresh tissue samples were obtained from 5 cases of normal ovaries, 6 cases of non-neoplastic disease, 21 cases of benign ovarian tumors, and 12 cases of malignant ovarian tumors. The expression of MAGE A1-6 and SSX 1-9 was detected by nested reverse transcription-polymerase chain reaction using each common primers sets for MAGE A1-6 and SSX 1-9.
Results: The expression rate of MAGE 1-6 mRNA was 23.0% (5/21) for the benign ovarian tumors and 91.7% (11/12) for the malignant ovarian tumor, whereas the normal ovaries (0/5) and non-neoplastic ovarian tissues (0/6) did not express MAGE (p<0.05). The expression rate of SSX was 40.0% (2/5) for the normal ovaries, 23.0% (5/21) for the benign ovarian tumors, and 33.3% (4/12) for the malignant ovarian tumors, while the non-neoplastic ovarian tissues showed no expression of SSX (p>0.05). A relationship between the two genes was not observed (kappa coefficient=0.32).
Conclusions: These results suggest that the gene products of MAGE and SSX can be useful for the immunotherapy of ovarian cancer patients and that MAGE can be a more promising target than SSX from the viewpoint of applicability and cancer-specificity.
키워드
Ovarian cancer;MAGE;SSX;Common primers
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