PTEN and p53 Mutations in Endometrial Carcinomas
최재성, 이윤이, 서광선, 나선영, 노홍태, 이혜경,
소속 상세정보
최재성 ( Choi Jae-Sung )
충남대학교 의과대학 산부인과학교실
이윤이 ( Rhee Yun-Ee )
충남대학교 의과대학 산부인과학교실
서광선 ( Suh Kwang-Sun )
충남대학교 의과대학 병리학교실
나선영 ( Na Sun-Young )
충남대학교 의과대학 병리학교실
노홍태 ( Noh Heung-Tae )
충남대학교 의과대학 산부인과학교실
이혜경 ( Lee Hye-Kyung )
가톨릭대학교 의과대학 대전성모병원 병리과학교실
KMID : 0357920050390010001
Abstract
Background: Endometrial carcinomas are pathogenetically classified into two major types; endometrioid carcinoma (EC) and serous carcinoma (SC). The most frequently altered gene in EC is the PTEN tumor suppressor gene (TSG). SC is usually associated with mutations in the p53 TSG.
Methods: To further determine the role of PTEN and p53 mutation in endometrial carcinogenesis, the analysis of 33 endometrial carcinomas, including 28 ECs and 5 SCs, for loss of heterozygosity (LOH) on 10q23 and for mutation in all 9 coding exons of PTEN and the 5-8 exons of p53, using SSCP-PCR methods was carried out.
Results: LOH was detected in at least one marker in 12 (54.5%) of 22 ECs, but in only one (20.0%) of 5 SCs. Somatic PTEN mutations were detected in 10 (35.7%) of 28 ECs. PTEN was altered in 67.9% of ECs and in 20.0% of SCs, including those with 10q23 LOH. No PTEN mutations were found among the SCs. Somatic p53 mutations were detected in 2 (7.1%) of 28 ECs and 3 (60.0%) of 5 SCs.
Conclusions: PTEN gene alterations contribute to the pathogenesis of an endometrioid subtype of endometrial carcinoma, but not to the serous type. In contrast, p53 plays an important role in the pathogenesis of SCs.
키워드
Carcinoma; endometrioid;PTEN gene product ;Genes; p53;Mutation;Loss of heterozygosity
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