한국인 희소돌기아교세포종의 1p/19q 이형접합소실 검사에 적합한 현미부수체 표지자
Adequate Microsatellite Markers for 1p/19q Loss of Heterozygosity of Oligodendroglial Tumors in Korean Patients
김세훈, 김태승, 김호근,
소속 상세정보
김세훈 ( Kim Se-Hoon )
연세대학교 의과대학 병리학교실
김태승 ( Kim Tai-Seung )
연세대학교 의과대학 병리학교실
김호근 ( Kim Ho-Guen )
연세대학교 의과대학 병리학교실
KMID : 0357920050390010023
Abstract
Background: It is well known that oligodendrogliomas can be divided into two groups according to the 1p/19q or 1p loss of heterozygosity (LOH) status because oligodendrogliomas with the 1p/19q LOH or the 1p LOH have a better prognosis and chemosensitivity. In this study, we investigated the adequate microsatellite markers for 1p/19q LOH of oligodendroglial tumors in Korean patients.
Methods: We performed PCR that was based on the LOH test with the 1p (D1S508, D1S199, D1S2734, D1S186 & D1S312) and 19q (D19S219, D19S112, D19S412 & D19S596) microsatellite markers; these were the markers that were recommended by other researchers. We performed this PCR on microdissected paraffin embedded tissue blocks of 67 tumors from 56 cases.
Results: The PCR based LOH analysis revealed that 3 microsatellite markers (D1S508, D1S2734 & D1S186) of 1p and 2 markers (D19S219 & D19S412) of 19q had higher heterozygosity scores than other markers. In addition, chromosomal LOH status using these selective markers showed a statistically significant difference of prognosis for oligodendroglial tumors.
Conclusions: We can suggest that the microsatellite markers with high heterozygosity scores (D1S508, D1S2734, D1S186, D19S219 and D19S412) would be adequate microsatellite markers for a PCR based LOH test of oligodendroglial tumors in Korean patients.
키워드
Oligodendroglioma;Chromosomes human pair 1;Chromosomes human pair 19;Loss ofheterozygosity;makers microsatellite
원문 및 링크아웃 정보
등재저널 정보