자궁내 발달지연과 정상 말기임신 태반에서의 Telomerase 활성도와 MB-1및 Fas, Fas Ligand 발현차이에 관한 연구
Telomerase activity and Expression of MIB-1, Fas and Fas Ligand in Placentas from Women with and without Intrauterine Growth Retardation
전이경, 양문호, 홍성란,
소속 상세정보
전이경 ( Chun Yi-Kyeong )
성균관대학교 의과대학 삼성제일병원 병리과
양문호 ( Yang Moon-Ho )
경희대학교 의과대학 병리학교실
홍성란 ( Hong Sung-Ran )
성균관대학교 의과대학 삼성제일병원 병리과
KMID : 0357920050390010034
Abstract
Background: The placenta from a pregnancy that is complicated by intrauterine growth retardation (IUGR) tends to be smaller than that from a normal pregnancy. To investigate this difference, we analyzed the telomerase activity, the proliferative activity and the mRNA levels of apoptosis mediators in placentas.
Methods: In 20 placentas from normal third-trimester pregnancies and 22 placentas form pregnancies that were complicated by IUGR, the telomerase activity was detected by a telomeric repeat amplification protocol assay. The proliferative activity was assessed by immunohistochemical staining using the MIB-1 monoclonal antibody. The expression of the apoptosis mediator was evaluated by semi-quantitative reverse transcription-polymerase chain reactions for fas and fas ligand.
Results: Telomerase activity was detected in 2 (10%) of 20 normal placentas, whereas it was not observed in all tested 13 placentas that were associated with IUGR. The proliferative activity was significantly low in the placentas that were associated with IUGR (7.44 2.96%), compared with the normal placentas (11.0 3.48%, p=0.002). There was no statistically significant difference in the mRNA levels of fas or fas ligand between two groups.
Conclusions: Low telomerase and proliferative activities in the placenta may play a role in the pathogenesis of IUGR.
키워드
Telomerase;MIB-1;Fas;Fas-ligand;Placenta
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