대장암에서 세포자멸사 유발성 bcl-2 Family 유전자의 돌연변이 연구
Mutational Analysis of Proapoptotic bcl-2 Family genes in Colon Carcinomas
송영화, 남석우, 유남진, 박원상, 이석형, 김수영, 이종우, 이정용,
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송영화 ( Song Young-Hwa )
가톨릭대학교 의과대학 병리학교실
남석우 ( Nam Suk-Woo )
가톨릭대학교 의과대학 병리학교실
유남진 ( Yoo Nam-Jin )
가톨릭대학교 의과대학 병리학교실
박원상 ( Park Won-Sang )
가톨릭대학교 의과대학 병리학교실
이석형 ( Lee Sug-Hyung )
가톨릭대학교 의과대학 병리학교실
김수영 ( Kim Su-Young )
가톨릭대학교 의과대학 병리학교실
이종우 ( Lee Jong-Woo )
가톨릭대학교 의과대학 병리학교실
이정용 ( Lee Jung-Young )
가톨릭대학교 의과대학 병리학교실
KMID : 0357920050390030168
Abstract
Background: Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. Members of the bcl-2 family proteins regulate the intrinsic apoptosis pathway mainly in the mitochondria. The aim of this study was to explore whether the somatic mutation of the proapoptotic bcl-2 family genes, one of the mechanisms that prolong the survival of cancer cells, occurred in colorectal carcinomas.
Methods: In the current study, to detect the somatic mutations in the DNA sequences encoding the bcl-2 homology 3 (BH3) domain of the human bak, bid, bik, bim, PUMA, bcl-rambo, bcl-G, and bmf genes in 98 colon adenocarcinomas, we used polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), and DNA sequencing.
Results: The SSCP analysis detected no evidence of somatic mutations of the genes in the coding regions of the BH3 domain in the cancers.
Conclusions: The data presented here indicate that the proapoptotic bcl-2 family genes, bak, bid, bik, bim, PUMA, bcl-rambo, bcl-G and bmf may not be somatically mutated in human colorectal carcinomas, and suggest that the colorectal cancers may not utilize mutational events of these proapoptotic bcl-2 family genes in the mechanisms for evading apoptosis.
키워드
Colonic neoplasms;Genes;bcl-2;Apoptosis;Mutation
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