간세포암종에서 Thrombospondin-1 및 -2의 발현: 종양 혈관신생 및 p53 과발현과의 연관성
Thrombospondin-1 and -2 Expressions in Hepatocellular Carcinomas: an Association with Tumor Angiogenesis and p53 Overexpression
정재신, 박호성, 손현진, 강명재, 문우성,
소속 상세정보
정재신 ( Chung Jae-Sin )
전북대학교 의과대학 병리학교실
박호성 ( Park Ho-Sung )
전북대학교 의과대학 병리학교실
손현진 ( Son Hyun-Jin )
전북대학교 의과대학 병리학교실
강명재 ( Kang Myoung-Jae )
전북대학교 의과대학 병리학교실
문우성 ( Moon Woo-Sung )
전북대학교 의과대학 병리학교실
KMID : 0357920050390040215
Abstract
Background: It has been suggested that thrombospondin (TSP) is a p53-dependent negative regulator of tumor angiogenesis. TSP expression and localization in hepatocellular carcinomas (HCCs) and its association with overexpression of p53 protein were investigated.
Methods: TSP-1 and -2 expressions were examined in 40 HCC specimens by immunohistochemical staining and in 4 HCC cell lines by Western blotting. In addition, p53 protein expression and microvessel density (MVD) were correlated with the TSP expression.
Results: Strong immu- nopositivity for TSP-1 was observed in fibroblasts, vascular endothelial cells, and some vas- cular smooth muscle cells of the stroma in 18 cases (45%), and in tumor cells in 3 cases (7.5%) of 40 cases of HCC. Immunoreactivity for TSP-2 was observed in only the sinusoidal lining cells of the tumor in 15 cases (46%), and in tumor cells in 2 cases (6%) of 32 cases of HCC. TSP-1 expression was inversely correlated with MVD (p=0.028), but TSP-2 expression did not show any correlation with MVD. Although p53 was overexpressed in 17 cases, there was no significant correlation between TSP and p53 expressions. None of the HCC cell lines expressed TSP-1 or -2.
Conclusions: These findings indicate that TSP-1 is mainly derived from nonparenchymal cells, and may decrease tumor angiogenesis in HCC.
키워드
Thrombospondins;Carcinoma; hepatocellular;Angiogenesis inhibitors;Protein; p53
원문 및 링크아웃 정보
등재저널 정보