Loss of PTEN Expression in Breast Cancers
장선희, 문병인, 서현숙, 성순희, 한운섭, 조민선, 이시내, 최혜영, 구혜수, 임석아,
소속 상세정보
장선희 ( Chang Sun-Hee )
인제대학교 의과대학 일산백병원 병리과
문병인 ( Moon Byung-In )
이화여자대학교 의과대학 외과학교실
서현숙 ( Suh Hyun-Suk )
이화여자대학교 의과대학 방사선종양학교실
성순희 ( Sung Sun-Hee )
이화여자대학교 의과대학 병리학교실
한운섭 ( Han Woon-Sup )
이화여자대학교 의과대학 병리학교실
조민선 ( Cho Min-Sun )
이화여자대학교 의과대학 병리학교실
이시내 ( Lee Shi-Nae )
이화여자대학교 의과대학 병리학교실
최혜영 ( Choi Hye-Young )
이화여자대학교 의과대학 영상의학교실
구혜수 ( Koo Hea-Soo )
이화여자대학교 의과대학 병리학교실
임석아 ( Im Seock-Ah )
이화여자대학교 의과대학 내과학교실
KMID : 0357920050390040236
Abstract
Background: PTEN, located on chromosome 10q23.31, is a novel tumor suppressor gene. In the sporadic breast cancers, the incidence of the loss of heterozygosity of PTEN is approximately 10% to 40%, but the incidence of intragenic mutation of PTEN is less than 1%. To as- sess the role of the PTEN in the invasive ductal breast cancer, we studied the frequency of the loss of PTEN expression, its correlation with the commonly used prognostic factors of the breast cancer and with PTEN promoter hypermethylation status.
Methods: Immunohistochemical staining with an anti-PTEN protein antibody was performed on the paraffin-embedded breast tissues from 129 women with a diagnosis of invasive ductal carcinoma. Methylation specific PCR was performed to detect hypermethylation in the PTEN gene on the 28 cases with the loss of PTEN expression.
Results: Sixty-two (48%) of 129 breast tumors had the loss of PTEN expression. The loss of PTEN expression was correlated with lymph node metastasis and stage, and there was a near-significant correlation with the tumor size. PTEN promoter hypermethylation was found in five (18%) out of 28 patients.
Conclusion: These results suggest that the loss of PTEN expression might play a role in the progression of the breast cancer and that the aberrant promoter methylation is one of the silencing mechanisms of PTEN.
키워드
PTEN;Invasive ductal carcinoma;Breast;Promoter hypermethylation
원문 및 링크아웃 정보
등재저널 정보