Expression of the 14-3-3 sigma Protein and Methylation Status of the 14-3-3 sigma gene in Biliary Neoplasms
송동은, 유은실, 심용희, 이승규, 박혜정, 이영주, 김명환, 이상수, 김순중, 장진세,
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송동은 ( Song Dong-Eun )
울산대학교 의과대학 서울아산병원 병리과
유은실 ( Yu Eun-Sil )
울산대학교 의과대학 서울아산병원 병리과
심용희 ( Shim Yhong-Hee )
건국대학교 Biological Science and Bio/Molecular Informatics Center
이승규 ( Lee Seung-Gyu )
울산대학교 의과대학 서울아산병원 일반외과
박혜정 ( Park Hae-Joung )
건국대학교 Biological Science and Bio/Molecular Informatics Center
이영주 ( Lee Young-Joo )
울산대학교 의과대학 서울아산병원 일반외과
김명환 ( Kim Myung-Hwan )
울산대학교 의과대학 서울아산병원 내과
이상수 ( Lee Sang-Soo )
울산대학교 의과대학 서울아산병원 내과
김순중 ( Kim Sun-Jung )
울산대학교 의과대학 서울아산병원 병리과
장진세 ( Jang Jin-Se )
울산대학교 의과대학 서울아산병원 병리과
KMID : 0357920060400010009
Abstract
Background: The 14-3-3 sigma ( ) protein has a negative regulatory role in the cell cycle progression of the. Down-regulation or overexpression of the 14-3-3 protein has been reported in various human cancers.
Methods: Immunohistochemistry for the 14-3-3 protein was performed in non-neoplastic bile duct cells, intraductal papillary neoplasms of the liver (IPNL), mass-forming intrahepatic cholangiocarcinomas (ICC) and non-papillary extrahepatic cholangiocarcinomas (ECC). We investigated the methylation status of the 14-3-3 gene in 45 cases of these 3 tumor groups.
Results: The non-neoplastic bile duct cells demonstrated negative or weakly positive cytoplasmic immunoreactivity for the 14-3-3 protein and no methylation of the 14-3-3 gene. Overexpression as well as negative immunoreactivity associated with hypermethylation of the 14-3-3 protein was observed in 16 (69.6%) of 23 cases of IPNL, in 21 (63.6%) of 33 cases of mass-forming ICC and in 27 (71.1%) of 38 cases of non-papillary ECC. Negative immunoreactivity was increased in the invasive IPNL (4/6, 66.7%), as well as in the poorly differentiated cases of mass-forming ICC (8/12, 66.7%) and the non-papillary ECC (5/8, 62.5%).
Conclusions: The similar rates for the abnormal expression of the 14-3-3 protein among the three groups of biliary neoplasms indicate its general association with biliary carcinogenesis. Furthermore, the loss of the 14-3-3 protein may be involved in the tumor progression and differentiation in the biliary carcinogenesis.
키워드
14-3-3 proteins;Bile ducts;Biliary tract neoplasms;Immunohistochemistry
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