Genistein과 Daidzein이 대장암 HCT-116 세포의 성장에 미치는 영향
Effects of Genistein and Daidzein on the Growth of Human Colon Cancer HCT-116 Cells
신종헌, 강구성, 김정옥, 김정완, 손윤경, 권태균, 윤길숙,
소속 상세정보
신종헌 ( Shin Jong-Heon )
경북대학교 의과대학 병리학교실
강구성 ( Kang Ku-Seong )
경북대학교 의과대학 병리학교실
김정옥 ( Kim Joung-Ok )
경북대학교 의과대학 병리학교실
김정완 ( Kim Jung-Wan )
경북대학교 치과대학 구강미생물학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
권태균 ( Kwon Tae-Gyun )
경북대학교 의과대학 비뇨기과학교실
윤길숙 ( Yun Kil-Suk )
경북대학교 의과대학 병리학교실
KMID : 0357920060400010046
Abstract
Background: Genistein and daidzein are two major soybean isoflavones. They have received increasing attention because of their possible roles for cancer prevention. However, their mechanisms of action and molecular targets on the human colon cancer cells are not fully understood.
Methods: Human colon cancer HCT-116 cells were treated with genistein and daidzein to investigate their effects on the cell growth and this was analyzed with MTT assay. TUNEL assay and Hoechst33342 stain were carried out to identify apotosis.
Results: Daidzein was able to inhibit cell proliferation and induce apoptosis of the HCT-116 cells, but genistein didn’t affect the cell growth. The ER antagonist ICI182780 didn’t attenuate the antiproliferative and proapoptotic effects of daidzein: this means the effect of daidzein on the HCT-116 cells may not be dependent on the ER pathway. The other soybean isoflavone, genistein, attenuated the effects of daidzein on the HCT-116 cells and its mechanism should be elucidated.
Conclusions: These data suggest that daidzein may act as a preventive agent on human colon cancer, and its mechanism of action doesn’t involve the ER-dependent pathway.
키워드
Genistein;Daidzein;Colon cancer;HCT-116 cell
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