Genetic Analysis of Epstein-Barr Virus Latent Membrane Protein 1 and Immunohistochemical Expression of Transforming Growth Factor (TGF)-β1, TGF-βRII, p21, p16, E2F1, Thymidylate Synthase, and NF-κB in Epstein-Barr Virus Encoded RNA-positive Gastric Adenoc
조미연, 김대성, 최선주, 엄민섭, 김명수, 박광하, Kim Mee-Dong, Sung Seung-Hoon,
소속 상세정보
조미연 ( Cho Mee-Yon )
연세대학교 원주의과대학 병리학교실
김대성 ( Kim Dae-Sung )
연세대학교 원주의과대학 외과학교실
최선주 ( Choi Sun-Ju )
연세대학교 원주의과대학 미생물학교실
엄민섭 ( Eum Min-Seob )
연세대학교 원주의과대학 병리학교실
김명수 ( Kim Myoung-Soo )
연세대학교 원주의과대학 외과학교실
박광하 ( Park Kwang-Hwa )
연세대학교 원주의과대학 병리학교실
( Kim Mee-Dong )
연세대학교 원주의과대학 병리학교실
( Sung Seung-Hoon )
연세대학교 원주의과대학 외과학교실
KMID : 0357920060400030176
Abstract
Background: Although clinicopathologic differences have been described between Epstein-Barr virus (EBV)-positive and negative gastric adenocarcinomas, the pathogenetic basis for these differences remains unclear. In this study, efforts were made to confirm that expression of EBV-latent membrane protein (LMP1) and immunohistochemical characteristics of EBV-positive gastric adenocarcinomas.
Methods: We investigated genomic deletion, and RNA & protein expression of the EBV-LMP1, as well as immunohistochemical protein expression of transforming growth factor (TGF)-β1, TGF-βRII, p21, p16, E2F1, thymidylate synthase, and NF-κB in relation to EBV positive gastric adenocarcinoma.
Results: A total of 38 Epstein-Barr Virus Encoded RNA-positive and 80 negative gastric carcinomas were examined. A 30 bp DNA deletion in the EBV-LMP1 gene, initiating at codon 342, was detected in 94.4% of EBV-positive cases. By RT-PCR and western blotting, EBV-LMP1 mRNA and protein expressions were absent in all cases, re- gardless of DNA deletion. No significant differences in TGF-β1, TGF-βRII, p21, NF-κB, E2F1, or thymidylate synthase expression were identified. However, the decreased expression of p16 was found in 84.2% of EBV-positive carcinomas, relative to only 57.5% of EBV-negative tumors (p=0.024).
Conclusion: EBV-LMP1 DNA deletion, mRNA and protein losses are highly prevalent in EBV-positive gastric adenocarcinoma among Korean patients, along with decreased p16 expression.
키워드
Stomach neoplasms;Epstein-Barr virus;EBV-Associated Membrane Antigen;Protein p16
원문 및 링크아웃 정보
등재저널 정보