Effects of Selective Cyclooxygenase-2 Inhibitor NS-398 Pretreatment on the Rat Spinal Cord after Contusion Injury
Cheong Hyeon-Dae, 성주경, 강구성, 김정옥, 김정완, 손윤경, Ham In-Suk, 박태인,
소속 상세정보
( Cheong Hyeon-Dae )
경북대학교 의과대학 신경외과학교실
성주경 ( Sung Joo-Kyung )
경북대학교 의과대학 신경외과학교실
강구성 ( Kang Ku-Seong )
경북대학교 의과대학 병리학교실
김정옥 ( Kim Joung-Ok )
경북대학교 의과대학 병리학교실
김정완 ( Kim Jung-Wan )
경북대학교 치과대학 구강미생물학교실
손윤경 ( Sohn Yoon-Kyung )
경북대학교 의과대학 병리학교실
( Ham In-Suk )
경북대학교 의과대학 신경외과학교실
박태인 ( Park Tae-In )
경북대학교 의과대학 병리학교실
KMID : 0357920060400040255
Abstract
Background: Secondary spinal cord injury (SCI) that follows an initial mechanical insult can exacerbate the overall damage, limit the restorative processes and eventually lead to an in- creased neurological deficit. We hypothesized that selective inhibition of cyclooxygenase-2 (COX-2) may decrease the delayed cell death, and so this will contribute to decreased level of the secondary injury.
Methods: The dorsal surface of the cord at the T9 level was subjected to weight drop impact using a 10 g rod. To block COX-2 activation, a selective COX-2 inhibitor (NS-398) was administered (5 mg/kg, i.p.) 15 min prior to SCI. The COX-1, COX-2, Caspase-3 and PGE2 expressions were measured by real time quantitative RT-PCR and fluorescence immunostaining.
Results: Many activated caspase-3 positive cells were observed at 6 h and they increased until 72 h after SCI. The expression of COX-2 peaked at 6 h after SCI, while the COX-1 expression was unaffected. The principal cells that showed a COX-2 expression were the neurons and microglia. Pretreatment with NS-398 caused a significant decrease in the expression of prostaglandin E2 and activated caspase-3 positive cells after SCI.
Conclusion: These data suggest that COX-2 is one of the main factors related with the pathologic deficits from secondary SCI.
키워드
Spinal cord injury;Cyclooxygenase 2 inhibitors;NS-398;Cell death
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