Assessment of Apoptosis by M30 Immunoreactivity and the Relationship with the MSI status and the Clinicopathological Characteristics of Colorectal Carcinomas
강현정, 설미영, 박도윤, 이수한, 신동훈, 김지연, 최경운, 김활웅, 이창훈, 허기영,
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강현정 ( Kang Hyun-Jeong )
부산대학교 의과대학 병리학교실
설미영 ( Sol Mi-Young )
부산대학교 의과대학 병리학교실
박도윤 ( Park Do-Youn )
부산대학교 의과대학 병리학교실
이수한 ( Lee Soo-Han )
부산대학교 의과대학 병리학교실
신동훈 ( Shin Dong-Hun )
부산대학교 의과대학 병리학교실
김지연 ( Kim Jee-Yeon )
부산대학교 의과대학 병리학교실
최경운 ( Choi Kyung-Un )
부산대학교 의과대학 병리학교실
김활웅 ( Kim Hwal-Woong )
부산대학교 의과대학 병리학교실
이창훈 ( Lee Chang-Hun )
부산대학교 의과대학 병리학교실
허기영 ( Huh Gi-Yeong )
부산대학교 의과대학 법의학교실
KMID : 0357920060400050319
Abstract
Background: The monoclonal antibody M30 recognizes a neoepitope of cytokeratin 18 that’s produced during the process of apoptosis, and it is reactive in formalin-fixed, paraffin-embedded tissue. The detailed nature of apoptosis in colorectal cancer is unclear, especially in regard to the MSI status and the clinicopathologic factors. The purpose of this study was to elucidate the apoptosis assessed by M30 immunoreactivity in colorectal cancer and its relationship with the MSI status and the various clinicopathologic factors of colorectal cancers.
Methods: 101 colorectal cancers were classified according to levels of MSI as 12 MSI-H, 4 MSI-L and 85 MSS. Apoptosis was quantified by immunohistochemistry with using M30 CytoDEATH antibody.
Results: The apoptotic index assessed by M30 was significantly increased in the MSI-H and MSI-L colorectal cancer compared to that in the MSS colorectal cancer. Right sided colon cancer showed a significant higher apoptotic index than did the left sided colon cancer. There was also a tendency for decreased apoptosis in metastatic colorectal cancers (Duke’s stage D). There was somewhat of an increase of apoptosis in colorectal cancers with mucinous carcinoma and medullary carcinoma, and also in the colorectal cancers with an increased TIL count, but this was not statistically significant.
Conclusion: M30 immunoreactivity is a valuable method to detect apoptosis in formalin-fixed, paraffin-embedded tissue and it might explain that MSI-H colorectal cancer shows better clinical behavior than MSS colorectal cancer in regard to the increased apoptosis.
키워드
M30;Apoptosis;Colorectal cancer;Microsatellite instability
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