N-butyl-N-(4-hydroxybutyl)nitrosamine으로 유도된 흰쥐의 방광암 발생과정에서 Survivin, HSP90, Bcl-2, Bax 단백의 발현
Expression of Survivin, HSP90, Bcl-2 and Bax Proteins in N-butyl-N- (4-hydroxybutyl)nitrosamine-induced Rat Bladder Carcinogenesis
이상대, 박성웅, 홍순억, 권귀영, 이태진,
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이상대 ( Lee Sang-Dae )
중앙대학교 의과대학 병리학교실
박성웅 ( Park Sung-Woong )
중앙대학교 의과대학 병리학교실
홍순억 ( Hong Soon-Auck )
중앙대학교 의과대학 병리학교실
권귀영 ( Kwon Gui-Young )
중앙대학교 의과대학 병리학교실
이태진 ( Lee Tae-Jin )
중앙대학교 의과대학 병리학교실
KMID : 0357920060400050333
Abstract
Background: Survivin belongs to the inhibitor of apoptosis family, and it has recently been found to be expressed in most solid tumors. Therefore, its expression is suggested to have prognostic significance. However, no data are available concerning the significance of survivin for the carcinogenesis of bladder cancer.
Methods: In order to induce urothelial tumor in the rat urinary bladder, 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) was administered to male Sprague-Dawley rats for 30 weeks. We used immunohistochemistry to investigate the expressions of survivin, HSP90, Bcl-2 and Bax in rat bladder carcinogenesis.
Results: Urothelial cell hyperplasia, papilloma, non-invasive urothelial carcinoma and invasive urothelial carcinoma appeared at 5, 10, 20 and 30 weeks, respectively. The expressions of survivin and HSP90 increased sequentially from normal mucosa, hyperplasia, papilloma, non-invasive urothelial carcinoma to invasive urothelial carcinoma. The expressions of Bcl-2 and Bax did not increase, however the number of cases with more than 1 of Bcl-2/Bax expression ratio increased sequentially during the progression of urothelial lesion. The expression of survivin showed a statistically significant correlation with the expression of HSP90 and the Bcl-2/Bax expression ratio.
Conclusions: Our findings suggest that survivin may be involved in the carcinogenesis of rat bladder and its expression is correlated with the expression of HSP90 and the Bcl-2/Bax expression ratio.
키워드
Urinary bladder;Carcinogenesis;Survivin;HSP90;Bcl-2;Bax
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