자궁경부 세포진 검사 이상과 자궁경부 종양 소견을 보인 환자에게서 제한효소질량다형성 분석과 DNA chip을 통한 인유두종 바이러스 유전자형 검사의 유용성 비교
Comparison of Efficacy of Human Papilloma Virus Genotyping Assays using Restriction Fragment Mass Polymorphism and DNA Chip Analysis in Patients with Abnormal Pap Smear and Uterine Cervical Cancer
정현재, 김성남, 이은희, 지미선, 김민아, 황선영, 조희정, 김수옥, 홍선표,
소속 상세정보
정현재 ( Chung Hyun-Jae )
(주)진매트릭스 중앙연구소
김성남 ( Kim Sung-Nam )
녹십자의료재단 진단검사의학과
이은희 ( Lee Eun-Hee )
녹십자의료재단 진단검사의학과
지미선 ( Jee Mi-Sun )
녹십자의료재단 진단검사의학과
김민아 ( Kim Min-A )
서울대학교 의과대학 병리학교실
황선영 ( Hwang Sun-Young )
(주)진매트릭스 중앙연구소
조희정 ( Cho Hee-Jung )
(주)진매트릭스 중앙연구소
김수옥 ( Kim Soo-Ok )
(주)진매트릭스 중앙연구소
홍선표 ( Hong Sun-Pyo )
(주)진매트릭스 중앙연구소
KMID : 0357920060400060439
Abstract
Background: High-risk human papilloma virus (HPV) infection is the primary cause of cervical cancer; there is a need for more sensitive and reliable methods for HPV genotyping to use as screening tools for early detection and intervention.
Methods: A novel MALDI-TOF MS-based assay, termed Restriction Fragment Mass Polymorphism (RFMP) was developed for multiple HPV genotyping. Its performance was compared with DNA chip technology. The study was based on 164 cases classified as normal (n=40), ASCUS (n=53) and invasive squamous cell carcinoma (SCC, n=71) by a PAP smear and/or cervical colposcopic biopsy.
Results: High-risk genotypes were detected in 7.5%, 47.2% and 97.2% in normal, ASCUS and SCC groups by RFMP, and in 20.0%, 41.5% and 90.1% using DNA chip technology, respectively. The results showed substantial concordance, with a kappa coefficient of 0.688, between the methods. Diagnostic sensitivity and specificity for cervical cancer were found to be 97.2% and 92.2% with RFMP and 90.1% and 80.0% using DNA chip microarrays.
Conclusions: RFMP and DNA chip technologies were shown to be reliable methods for HPV genotyping with a high concordance. The improved sensitivity and specificity should make RFMP a viable option for the management of women with cervical neoplastic lesions.
키워드
Human papillomavirus;Genotype;Matrix-assisted laser desorption-ionization mass spectrometry
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