자궁목암종에서 Tetranucleotide Repeat Microsatellite Instability에 관한 연구
Tetranucleotide Repeat Microsatellite Instability in Uterine Cervical Carcinomas
최유덕, 이지신, 최찬, 박창수, 정상우, 남종희, 최호선,
소속 상세정보
최유덕 ( Choi Yoo-Duk )
전남대학교 의과대학 병리학교실
이지신 ( Lee Ji-Shin )
전남대학교 의과대학 병리학교실
최찬 ( Choi Chan )
전남대학교 의과대학 병리학교실
박창수 ( Park Chang-Soo )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
남종희 ( Nam Jong-Hee )
전남대학교 의과대학 병리학교실
최호선 ( Choi Ho-Sun )
전남대학교 의과대학 산부인과학교실
KMID : 0357920070410010030
Abstract
Background : Elevated levels of microsatellite alterations at selected tetranucleotide repeat regions (EMAST) have been recently described, and they are a distinct type of microsatellite instability (MSI). We investigated the prevalence of EMAST in squamous cell carcinoma (SCC) of the uterine cervix and we determined the correlation between EMAST and the clinicopathologic parameters, HPV infection and the p53 mutation.
Methods : We examined the 3 mono-, 3 di-, and 5 tetranucleotide repeat markers in 47 cases of SCC, and we performed immunohistochemical staining for p53. HPV detection and genotyping was performed using a commercially available HPV DNA chip.
Results : Thirteen out of 47 cases (27.7%) were EMAST(+) with at least one of five tetranucleotide repeat markers. However, MSI at mono- and dinucleo- tide markers was noted in only one case (2.1%). EMAST was not related with stage, size, lymph node metastasis, vascular/lymphatic invasion or the depth of invasion. Positive immunostaining for p53 was significantly more common in EMAST(+) tumors than in the EMAST(-) tumors (p=0.04). HPV-infection was positive in 32 cases. EMAST was not correlated with the state of HPV infection state or the HPV genotype.
Conclusions : 27.7% of the invasive SCCs of the uterine cervix exhibited EMAST, and EMAST in the SCC of the uterine cervix was significantly associated with the p53 mutation.
키워드
Cervix; Squamous cell carcinoma; Microsatellite instability; Human papillomavirus; p53
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