Immunohistochemical Analysis of Midkine Expression in Preinvasive and Invasive Squamous Cell Neoplasia of the Uterine Cervix
최석진, 송은섭, 김루시아, 한지영, 김준미, 주영채, 박인서,
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최석진 ( Choi Suk-Jin )
인하대학교 의과대학 병리학교실
송은섭 ( Song Eun-Seop )
인하대학교 의과대학 산부인과학교실
김루시아 ( Kim Lucia )
인하대학교 의과대학 병리학교실
한지영 ( Han Jee-Young )
인하대학교 의과대학 병리학교실
김준미 ( Kim Joon-Mee )
인하대학교 의과대학 병리학교실
주영채 ( Chu Young-Chae )
인하대학교 의과대학 병리학교실
박인서 ( Park In-Suh )
인하대학교 의과대학 병리학교실
KMID : 0357920070410020079
Abstract
Background: Midkine (MK) is a member of the heparin-binding growth factor family. Overexpression of MK is observed not only in cancerous tissue but also in precancerous lesions of the colon and the prostate. Using immunohistochemical methods, we investigated MK expression in preinvasive and invasive neoplasia of the uterine cervix.
Methods: We performed im- munohistochemical analysis of archived cone biopsy and hysterectomy specimens from 161 squamous cell lesions of the uterine cervix (29 cervical intraepithelial neoplasia 1 (CIN1), 35 CIN2, 49 CIN3, 30 microinvasive squamous cell carcinomas (MIC), and 18 invasive squamous cell carcinoma). In addition, we examined if there is a correlation between MK expression and status of human papilloma virus infection determined by a commercially available DNA chip.
Results: None of the normal cervical mucosa showed MK immunostaining. The level of MK expression gradually increased according to the histologic grade. Moderate and strong expressions were most frequently observed in cervical tissue with CIN3 and MIC. MK immunostaining was more accentuated in the invasive border of MIC.
Conclusion: MK may play a functional role in the disease progression of cervical squamous cell neoplasia.
키워드
Cervical intraepithelial neoplasia; Midkine; Immunohistochemistry; Human papillomavirus
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