대장암에서 Cyclin-Dependent Kinase-Associated Protein Phosphatase 단백의 발현
Expression of Cyclin-Dependent Kinase-Associated Protein Phosphatase in Colorectal Carcinomas
김창남, 김주헌, 박미자, 강동욱, 김재화, 김수영, 손현진, 이혜경,
소속 상세정보
김창남 ( Kim Chang-Nam )
을지대학교 의과대학 외과학교실
김주헌 ( Kim Ju-Hun )
을지대학교 의과대학 병리학교실
박미자 ( Park Mi-Ja )
을지대학교 의과대학 병리학교실
강동욱 ( Kang Dong-Wook )
을지대학교 의과대학 병리학교실
김재화 ( Kim Jae-Wha )
한국생명공학연구원 세포체연구단
김수영 ( Kim Soo-Young )
을지대학교 의과대학 예방의학교실
손현진 ( Son Hyun-Jin )
을지대학교 의과대학 병리학교실
이혜경 ( Lee Hye-Kyung )
을지대학교 의과대학 병리학교실
KMID : 0357920070410060367
Abstract
Background : Cyclin-dependent kinase-associated phosphatase (KAP) is a human dual-specificity protein phosphatase that dephosphorylates Cdk2 on threonine160 in a cyclin-dependent manner and that is known as an up-regulated molecule in some malignant tumors. We investigated the expression and clinicopathologic significance of KAP protein in relation to tumorigenesis of colorectal carcinoma.
Methods : The expression patterns of KAP protein in tumor tissue were examined by reverse transcription-PCR and immunohistochemical staining.
Results :An enhanced transcriptional level of KAP mRNA was observed in 11 out of 12 colorectal carcinoma
specimens. Immunohistochemical examination showed that KAP protein was more highly expressed in the tumors than that in the adjacent non-neoplastic mucosal tissues for 52 of 102 colorectal cancer tissues. The statistical analysis showed that an increased level of KAP protein in the colorectal cancer tissues was inversely correlated with the histologic grade, tumor size and Duke’s stage.
Conclusion : The present study suggests that alteration of KAP might play a role, at least in part, in the tumorigenicity of colorectal carcinoma through the mechanism of cell cycle regulation.
키워드
KAP;Colorectal cancer;Immunohistochemistry
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