난소 상피암에서 현미부수체 불안정성 분석
Analysis of Microsatellite Instability in Ovarian Epithelial Cancer
설미영, 김지연, 신동훈, 최경운, 강현정, 김익두, 서순정, 최효선,
소속 상세정보
설미영 ( Sol Mi-Young )
부산대학교 의과대학 병리학교실
김지연 ( Kim Jee-Yeon )
부산대학교 의과대학 병리학교실
신동훈 ( Shin Dong-Hoon )
부산대학교 의과대학 병리학교실
최경운 ( Choi Kyung-Un )
부산대학교 의과대학 병리학교실
강현정 ( Kang Hyun-Jeong )
부산대학교 의과대학 병리학교실
김익두 ( Kim Ick-Doo )
부산대학교 의과대학 병리학교실
서순정 ( Seo Soon-Jung )
이수앱지스
최효선 ( Choi Hyo-Seon )
이수앱지스
KMID : 0357920070410060380
Abstract
Background : The aim of this study was to clarify the incidence and role of microsatellite instability (MSI) in sporadic ovarian epithelial cancers (OEC). We investigated the MSI status and mismatch repair (MMR) protein expression in OEC.
Methods : MSI was examined by fluorescence- based polymerase chain reaction using five NCI panel markers (BAT25, BAT26, D2S123, D5S346 and D17S250) in 46 cases of OEC. Immunohistochemistry (IHC) for hMLH1 and
hMSH2 was performed.
Results : Seven cases (15.2%) exhibited high-frequency MSI (MSIH), one exhibited low-frequency MSI (MSI-L), and the remaining 38 demonstrated microsatellite stability (MSS). MSI-H in OEC was not associated with histologic grade, FIGO stage, tumor size, mitoses or histologic type. Loss of expression of either hMLH1 or hMSH2 was observed
in 4 of the 7 (59.3%) MSI-H cases, whereas 4 of the 39 (10.3%) MSI-L or MSS tumors revealed loss of expression of MMR proteins. The sensitivity and specificity of immunohistochemistry for hMLH1 and hMSH2 were 57.1% and 89.7%.
Conclusions : Our data suggest that a genetic defect in the MMR system might play a role in the carcinogenesis of a minor subset of sporadic OEC however, immunohistochemical testing for hMLH1 and hMSH2 cannot accurately
determine microsatellite instability status in OEC.
키워드
Microsatellite instability;Ovarian neoplasm;hMLH1;hMSH2;Immunohistochemistry
원문 및 링크아웃 정보
등재저널 정보