흰쥐의 4-혈관폐쇄를 위한 간단하고 저렴한 앞목접근법 - 해마의 면역조직학적 변화와 Aminoguanidine이 신경세포사에 미치는 영향에 대한 연구 -
Anterior Neck Approach for 4-vessel Occlusion in Rats - A Study for Immunohistological Changes and the Effects of Aminoguanidine on Neuronal Cell Death in the Hippocampus -
안영준, 김용석, 최기용, 강구, 이승구, 정일영, 김근우,
소속 상세정보
안영준 ( Ahn Young-Jun )
강원대학교 의과대학 병리학교실
김용석 ( Kim Yong-Seok )
중앙대학교 의과대학 외과학교실
최기용 ( Choi Ki-Young )
강원대학교 의과대학 병리학교실
강구 ( Kang Gu )
강원대학교 의과대학 병리학교실
이승구 ( Lee Seung-Koo )
강원대학교병원 임상의학연구소
정일영 ( Cheong Il-Young )
강원대학교병원 임상의학연구소
김근우 ( Kim Keun-Woo )
강원대학교병원 임상의학연구소
KMID : 0357920070410060393
Abstract
Background : Clinically relevant cerebral ischemia is encountered most frequently as a cardiac arrest or as single or multiple occlusions of the intracranial or extracranial cerebral arteries. 1 Yamaguchi et al.2 has introduced a one-stage anterior approach to occlude the common carotid arteries (CCAs) and vertebral arteries (VAs).
Methods : We used a 2-stage anterior approach for producing transient global ischemia by 4-vessel occlusion (4-VO). Four to five days after electrocauterization of two VAs using the anterior neck approach, two CCAs were clipped for 10 min under anesthesia. Aminoguanidine (100 mg/kg) was administered intraperitoneally immediately after 4-VO, and then twice a day for three consecutive days. Cresyl violet staining and immunohistochemical analysis for the expression of GFAP, CD11b, nitrotyrosine, iNOS, and Bax were performed, using brain slices obtained from the rats that were sacrificed 1, 3, 5 and 7 days after reperfusion.
Results : Aminoguanidine reduced neuronal cell death in the CA1 region of the hippocampus. Expression of GFAP, CD11b, nitrotyrosine, iNOS, and Bax were significantly increased in the CA1 region of the hippocampus three days after 4-VO.
Conclusions : We believe that modified 4-VO is a good method to study transient forebrain ischemia as it is simple and inexpensive to perform and can be utilized without stereotaxis, a pivoting dissection microscope, EEG, a laser flowmeter or the use of Mongolian gerbils.
키워드
Brain ischemia;Rat;Aminoguanidine
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