Diffuse Large B Cell Lymphoma Shows Distinct Methylation Profiles of the Tumor Suppressor Genes among the Non-Hodgkin’s Lymphomas
윤선옥, 강경훈, 김영아, 김지은, 전윤경, 김철우,
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윤선옥 ( Yoon Sun-Och )
서울대학교 의과대학 병리학교실
강경훈 ( Kang Gyeong-Hoon )
서울대학교 의과대학 병리학교실
김영아 ( Kim Young-A )
서울대학교 의과대학 보라매병원 병리과
김지은 ( Kim Ji-Eun )
서울대학교 의과대학 보라매병원 병리과
전윤경 ( Jeon Yoon-Kyung )
서울대학교 의과대학 병리학교실
김철우 ( Kim Chul-Woo )
서울대학교 의과대학 병리학교실
KMID : 0357920080420010016
Abstract
Background : Aberrant methylation of CpG islands in promoter regions is one of the major mechanisms for silencing of tumor suppressor genes in various types of human cancers including non-Hodgkin’’ lymphomas (NHL). In this study, we investigated the aberrant promoter methylation status of known or suspected tumor suppressor genes in NHLs and compared the methylation profiles between B-cell and T/NK-cell NHLs.
Methods : 54 cases of B-cell NHLs and 16 cases of T/NK-cell NHLs were examined for the methylation status of eight genes using methylation specific PCR.
Results : CpG islands methylation was variously found in eight genes as follows; DAPK (71%), MT1G (70%), p16 (53%), CDH1 (53%), THBS1 (56%), MGMT (27.1%), COX2 (13%), and RUNX3 (11.4%). In six cases (8 %), methylation was not observed in any of these genes. Overall methylation index of B-cell NHLs (0.48) was significantly higher than that of T/NK-cell NHLs (0.32). Of eight genes tested, THBS1 and CDH1 methylations were much more prominent in diffuse large B-cell lymphomas than in T/NK-cell NHLs or other B-cell NHLs.
Conclusion : This study suggests that aberrant CpG island methylation is a frequent event in NHLs, and diffuse large B-cell lymphomas show overlapping but distinct methylation profiles.
키워드
Lymphoma; non-Hodgkin; DNA methylation; Genes; tumor suppressor
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