The Expression of Transforming Growth Factor-β1 and α-Smooth Muscle Actin is Increased in the Human Myxomatous Valve
박정환, 윤호중, Yoon Jung-Sook, 박철수, Oh Soo-Sung, 정우백, 정종원, 최윤석, 이동현, 오용석, 정욱성, 홍순조, 심성보, 이선희, 이연수,
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박정환 ( Park Jeong-Hwan )
가톨릭대학교 의과대학 내과학교실
윤호중 ( Youn Ho-Joong )
가톨릭대학교 의과대학 내과학교실
( Yoon Jung-Sook )
가톨릭대학교 의과대학 내과학교실
박철수 ( Park Chul-Soo )
가톨릭대학교 의과대학 내과학교실
( Oh Soo-Sung )
가톨릭대학교 의과대학 내과학교실
정우백 ( Chung Woo-Baek )
가톨릭대학교 의과대학 내과학교실
정종원 ( Chung Jong-Won )
가톨릭대학교 의과대학 내과학교실
최윤석 ( Choi Yun-Seok )
가톨릭대학교 의과대학 내과학교실
이동현 ( Lee Dong-Hyeon )
가톨릭대학교 의과대학 내과학교실
오용석 ( Oh Yong-Seog )
가톨릭대학교 의과대학 내과학교실
정욱성 ( Chung Wook-Sung )
가톨릭대학교 의과대학 내과학교실
홍순조 ( Hong Soon-Jo )
가톨릭대학교 의과대학 내과학교실
심성보 ( Sim Sung-Bo )
가톨릭대학교 의과대학 성모병원 흉부외과학교실
이선희 ( Lee Sun-Hee )
가톨릭대학교 의과대학 흉부외과학교실
이연수 ( Lee Youn-Soo )
가톨릭대학교 의과대학 병리학교실
KMID : 0357920090430020152
Abstract
Background: In vitro experimental studies have reported that transforming growth factor-β1 (TGF-β1) stimulates the production of α-smooth muscle actin (α-SMA) in porcine valves. However, the relation between TGF-β1 and α-SMA in myxomatous valves has not been elucidated.
Methods: We classified 27 subjects into two groups: 1) myxomatous group (M:F=11:12, mean age=55±15 years) and 2) rheumatic group (M:F=3:1, mean age=41±17 years) according to preoperative echocardiographic and postoperative histologic findings. Twenty-seven valve specimens from the patients who underwent valve replacement were obtained. Tissue samples were analyzed by immunohistochemistry for TGF-β1 and α-SMA. The positively stained areas were measured using an image analysis program (Image Pro-Plus 4.5), and then the TGF-β1 volume fraction (TGF-VF) and α-SMA volume fraction (α-SMA-VF) were calculated.
Results: TGF-VF in myxomatous valves was higher than in rheumatic valves (2,759±2,294 vs 864±276, p=0.04). α-SMA-VF in myxomatous valves was higher than in rheumatic valves (4,122±2,275 vs 2,421±844, p=0.002). There was a significant correlation between TGF-β1 and α-SMA in myxomatous valves (r=0.38, p=0.04). There was no significant correlation between TGF-β1 and α-SMA in rheumatic valves (r=-0.50, p=0.67).
Conclusions: TGF-β1 and α-SMA may be related to the pathogenesis of myxomatous valves. The activation of TGF-β1 might increase the expression of α-SMA in human myxomatous valves.
키워드
Transforming growth factor-beta1;Alpha-Smooth muscle actin;Myxomatous valve
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