뇌척수액에서 백혈병세포의 세포소견; 액상세포검사법과
The Cytology for Leukemic Cells in Cerebrospinal Fluid; Comparison of Conventional Cytology with Liquid-Based Cytology
유창영, 이연수, 강창석, 심상인, 이교영,
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유창영 ( Yoo Chang-Young )
가톨릭대학교 의과대학 병원병리학교실
이연수 ( Lee Youn-Soo )
가톨릭대학교 의과대학 병원병리학교실
강창석 ( Kang Chang-Suk )
가톨릭대학교 의과대학 병원병리학교실
심상인 ( Shim Sang-In )
가톨릭대학교 의과대학 병원병리학교실
이교영 ( Lee Kyo-Young )
가톨릭대학교 의과대학 병원병리학교실
KMID : 0357920090430020164
Abstract
Background: The cytological examination of cerebrospinal fluid (CSF) using conventional cytology with a cytocentrifuge (cytospin) is an important method for evaluating the involvement of leukemia in the CNS. Liquid-based cytology (LBC) is now a widely used cytological method not only for gynecological and non-gynecological specimens, but its application to CSF for the identification of leukemic cell has not yet been reported. In this study, we tried to compare conventional cytology with using a cytospin with LBC and Papanicolaou (Pap) staining. We also examined the modified LBC with Wright staining to assess whether this modified method can be useful for diagnosing Leukemia.
Methods: We studied 30 cases of CSF that were obtained from 16 patients, including 17 cases of acute myeloid leukemia, 12 cases of acute lymphoblastic leukemia and 1 case of diffuse large B cell lymphoma. We applied conventional cytology with a cytocentrifuge (cytospin), LBC with Pap staining and modified LBC with Wright staining.
Results: The morphological features of the LBC with Pap staining showed difficulty for interpretation when compared with conventional cytology with a cytospin, and mainly because of cellular shrinkage. The modified LBC with Wright staining showed good morphological features.
Conclusions: We suggest that modified LBC with Wright staining may be useful for examining CSF.
키워드
Cerebrospinal fluid;Liquid-based cytology;Cytospin;Wright staining;Papanico-laoustaining
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