Loss of Heterozygosity on Chromosome 15q15 Near Thrombospondin-1 Gene in Breast Carcinomas
김진아, 이희정, 유문간, 김경미, 이안희, 강석진, 이교영,
소속 상세정보
김진아 ( Kim Jean-A )
가톨릭대학교 의과대학 병리학교실
이희정 ( Lee Hee-Jeong )
가톨릭대학교 의과대학 병리학교실
유문간 ( Rhyu Mun-Gan )
가톨릭대학교 의과대학 미생물학교실
김경미 ( Kim Kyoung-Mee )
성균관대학교 의과대학 삼성서울병원 병리학교실
이안희 ( Lee Anhi )
가톨릭대학교 의과대학 병리학교실
강석진 ( Kang Seok-Jin )
가톨릭대학교 의과대학 병리학교실
이교영 ( Lee Kyo-Young )
가톨릭대학교 의과대학 병리학교실
Abstract
Background: Chromosome 15q15 near the thrombospondin-1 (THBS-1) gene may be associated with tumor progression and metastasis. To clarify the potential role of the15q15 region in progression of breast carcinoma, we investigated the loss of heterozygosity (LOH) and the microsatellite instability (MSI) status of chromosome 15q15.
Methods: LOH and MSI were detected in 84 breast carcinoma specimens using PCR-based microsatellite analysis with three microsatellite markers.
Results: Of 77 breast carcinomas containing the heterozygous alleles, 25 (32%) showed LOH in at least one microsatellite marker. Partial LOH and total LOH were detected in 14 (18.27%) and 11 (14.3%) cases. The total LOH were inversely correlated with node metastasis. A single LOH at D15S514 was inversely correlated with nuclear grade and a single LOH at the D15S129 allele was associated with increased expression of the THBS-1 gene. MSI-positive breast carcinomas detected in 14 (17%) cases showed no correlation with any clinicopathologic feature.
Conclusions: These results indicate that loss of the chromosome 15q15 region delays the progression of breast carcinoma because the magnitude of LOH is large and involves the THBS-1 gene and additional genetic elements. The genes located on chromosome 15q15 probably play a tissue-type-dependent role in malignant growth of the tumor.
키워드
Breast neoplasm;Thrombospondin-1;Loss of heterozygosity;Microsatellite instability
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