Analysis of HPV-other Samples by Performing HPV DNA Sequencing
최유덕, 한창우, Chung Woon-Jae, 정운원, 이지신, 남종희, 이민철, 정상우, 최호선, 박창수,
소속 상세정보
최유덕 ( Choi Yoo-Duk )
전남대학교 의과대학 병리학교실
한창우 ( Han Chang-Woo )
전남대학교 의과대학 병리학교실
( Chung Woon-Jae )
전남대학교 의과대학 병리학교실
정운원 ( Jung Woon-Won )
MyGene Bioscience Institute
이지신 ( Lee Ji-Shin )
전남대학교 의과대학 병리학교실
남종희 ( Nam Jong-Hee )
전남대학교 의과대학 병리학교실
이민철 ( Lee Min-Cheol )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
최호선 ( Choi Ho-Sun )
전남대학교 의과대학 산부인과학교실
박창수 ( Park Chang-Soo )
전남대학교 의과대학 병리학교실
KMID : 0357920090430030250
Abstract
Background: HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.
Methods: For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high- risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV- other samples of 209 patients who subsequently underwent cervical biopsy.
Results: For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.
Conclusions: HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.
키워드
DNA chips;Papillomaviridae;Sequence analysis;Uterine cervix
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