DNA Methylation Profiles of MGMT,DAPK1,hMLH1,CDH1,SHP1, and HIC1 in B-Cell Lymphomas
김성순, 최영표, 한창우, 최유덕, 이지신, 정상우, 최찬, 박영규, 이제중, 김형준, 이일권,
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김성순 ( Kim Sung-Sun )
전남대학교 의과대학 병리학교실
최영표 ( Choi Young-Hyo )
전남대학교 의과대학 병리학교실
한창우 ( Han Chang-Woo )
전남대학교 의과대학 병리학교실
최유덕 ( Choi Yoo-Duk )
전남대학교 의과대학 병리학교실
이지신 ( Lee Ji-Shin )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
최찬 ( Choi Chan )
전남대학교 의과대학 병리학교실
박영규 ( Park Young-Kyu )
전남대학교 의과대학 외과학교실
이제중 ( Lee Je-Jung )
전남대학교 의과대학 내과학교실
김형준 ( Kim Hyeoung-Joon )
전남대학교 의과대학 내과학교실
이일권 ( Lee Il-Kwon )
전남대학교병원 조혈계질환 유전체 연구센터
KMID : 0357920090430050420
Abstract
Background: This study was designed to examine the prevalence of aberrant promoter methylation in a selected panel of genes potentially involved in lymphoid tumors.
Methods: The promoter hypermethylation status of MGMT,DAPK1,hMLH1,CDH1,SHP1, and HIC1 was measured by methylation-specific PCR for 82 cases of B-cell lymphoma. Immunohistochemical staining using MGMT and SHP1 antibodies was conducted on 43 out of 82 cases.
Results: The number of MGMT aberrant methylations was lower in diffuse large B-cell lymphoma (DLBCL) than in other malignant lymphomas. The methylation of DAPK1 was frequently detected in follicular lymphoma (FL), marginal zone B-cell lymphoma (MZL) and DLBCL. With one exception, methylation of hMLH1 was not observed in B-cell lymphomas. The methylation frequency of CDH1, and HIC1 was similar in B-cell lymphomas. However, the methylation of SHP1 gene was more frequently observed in cases of FL, DLBCL, and MZL than in chronic lymphocytic lymphoma. MGMT and SHP1 promoter methylation were inversely correlated with the protein expression observed upon immunohistochemical staining.
Conclusions: Aberrant promoter methylation of multiple genes occurs with variable frequency throughout the B-cell lymphomas, and methylation of hMLH1 is rarely observed in B-cell lymphomas.
키워드
Lymphoma;Methylation;B-lymphocytes
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