Histopathological Evaluation of Pediatric Intestinal Pseudo-Obstruction: Quantitative Morphometric Analysis of Pathological Changes in the Enteric Nervous System
김현경, 정하린, 강한나, 배지윤, 송동은, 조민선, 성순희, 한운섭, 구혜수,
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김현경 ( Kim Hyung-Kyung )
이화여자대학교 의학전문대학원 병리학교실
정하린 ( Cheong Ha-Rin )
이화여자대학교 의학전문대학원 병리학교실
강한나 ( Kang Han-Na )
이화여자대학교 의학전문대학원 병리학교실
배지윤 ( Bae Ji-Yoon )
이화여자대학교 의학전문대학원 병리학교실
송동은 ( Song Dong-Eun )
이화여자대학교 의학전문대학원 병리학교실
조민선 ( Cho Min-Sun )
이화여자대학교 의학전문대학원 병리학교실
성순희 ( Sung Sun-Hee )
이화여자대학교 의학전문대학원 병리학교실
한운섭 ( Han Woon-Sup )
이화여자대학교 의학전문대학원 병리학교실
구혜수 ( Koo Hae-Soo )
이화여자대학교 의학전문대학원 병리학교실
KMID : 0357920100440020162
Abstract
Background: This study was done to obtain comprehensive data on changes in the structural components of the enteric nervous system in pediatric patients with intestinal pseudo-obstruction (IPO). We evaluated routinely processed, in formalin-fixed tissues by quantitative morphometric analysis. In addition, we used formalin-fixed tissue to explore the possibility of using previously proposed diagnostic criteria to evaluate frozen serial sections for intestinal neuronal dysplasia (IND) type B and hypoganglionosis.
Methods: We analyzed data for 19 IPO cases. Morphometric analysis for quantification of ganglia and ganglion cells (GCs) was done for the myentric and the submucous plexus. In addition, we determined the presence of immature GCs and the distribution of nerve fibers and interstitial cells of Cajal (ICC).
Results: Nine patients showed combined hypoganglionosis, IND, and decreased ICC; others showed various combinations of these. Several morphometric factors were significantly different between patient groups as well as being different than the control group.
Conclusions: Our pediatric IPO cases showed extensive overlapping of pathological findings. And the findings suggest the utility of using previously proposed morphometrically measured factors in multiple frozen sections as diagnostic criteria for IND type B and hypoganglionosis in formalin-fixed tissue.
키워드
Intestinal pseudo-obstruction;Morphometric analysis;Quantitative
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