세포검사로 유두갑상샘암종이 의심되는 갑상샘 결절의 Galectin-3 발현과 BRAF 돌연변이 분석
Galectin-3 Expression and BRAF Mutation in Cases of Cytologically Suspicious Papillary Thyroid Carcinoma
김도경, 김현지, 곽진영, 김민주, 정형재, 구자승, 임범진, 정찬권, 홍순원,
소속 상세정보
김도경 ( Kim Do-Kyung )
연세대학교 의과대학 병리학교실
김현지 ( Kim Hyun-Gi )
연세대학교 의과대학 병리학교실
곽진영 ( Kwak Jin-Young )
연세대학교 의과대학 영상의학교실
김민주 ( Kim Min-Ju )
연세대학교 의과대학 병리학교실
정형재 ( Jung Hyung-Jae )
연세대학교 의과대학 병리학교실
구자승 ( Koo Ja-Seung )
연세대학교 의과대학 병리학교실
임범진 ( Lim Beom-Jin )
연세대학교 의과대학 병리학교실
정찬권 ( Jung Chan-Kwon )
가톨릭대학교 의과대학 서울성모병원 병리과
홍순원 ( Hong Soon-Won )
연세대학교 의과대학 병리학교실
KMID : 0357920100440020191
Abstract
Background: Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions.
Methods: Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme.
Results: All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity.
Conclusions: This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.
키워드
Suspicious cytology;Biopsy;fine needle;BRAF mutation;Galectin-3
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