전이성 대장암종에서 Osteopontin 및 MMP-1의 유전자 발현 변화 및 연관 임상 소견 검증
Validation of Gene Expression Changes of Osteopontin and MMP-1 in Primary and Metastatic Colorectal Carcinomas
최준정, 김상겸, 박전한, 김남규, 김호근,
소속 상세정보
최준정 ( Choi Jun-Jeong )
연세대학교 의과대학 병리학교실
김상겸 ( Kim Sang-Kyum )
연세대학교 의과대학 병리학교실
박전한 ( Park Jeon-Han )
연세대학교 의과대학 외과학교실
김남규 ( Kim Nam-Kyu )
연세대학교 의과대학 미생물학교실
김호근 ( Kim Ho-Guen )
연세대학교 의과대학 병리학교실
KMID : 0357920100440030225
Abstract
Background: Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited.
Methods: Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays.
Results:
Conclusions: Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.
키워드
Neoplasm metastasis;Colorectal neoplasms;Immunohistochemistry;Osteopontin;Matrix metalloproteinase 1
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